FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775161
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCC27190.3507658409027286No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26580.34289650795125143No Hit
GATTATTACTGTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGG24200.31219320889466834No Hit
GCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCA23730.3061299523582843No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCT20720.2672993094337821No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATGGCCAGGGAGG18300.2360799885443153No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15580.20099050390822035No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCAC15430.1990554220349063No Hit
GTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCAC15140.1953142637464991No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14140.1824137179244054No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13920.17957559784354477No Hit
GTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA13340.1720932812667304No Hit
CCCCAGGGACGGCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCC13330.17196427580850945No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC12240.15790268086242729No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11650.15029135882739197No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTC11410.14719522783008948No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG11400.14706622237186856No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT11140.14371208045812417No Hit
CCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG11120.1434540695416823No Hit
ACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGG10750.1386808675875076No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10490.13532672567376325No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10200.13158556738535607No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGG9620.1241032508085417No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9500.12255518530989046No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG9240.11920104339614608No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9230.11907203793792515No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGT9000.1161049123988436No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8810.11365380869264578No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA8790.11339579777620391No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCT8550.11029966677890142No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT8300.10707453032337798No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8230.10617149211583142No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8190.10565547028294768No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8100.10449442115895924No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8060.1039783993260755No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCAC8000.10320436657674988No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7930.1023013283692033No Hit
GGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG7860.10139829016165673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10600.041.2801781
GTGGTAT2100.041.017051
TCGAATC350.00124126740.0928765
TGGTATC2200.037.579262
GGTATCA4600.036.7013471
TATCAAC12100.035.3017272
ATCAACG13250.032.4977873
TCAACGC13300.032.1166084
CAACGCA13600.031.4081555
AATGTTG11350.030.95536870
AACGCAG14000.030.5107786
CGCAGAG15400.028.1989978
ACGCAGA15400.027.9679767
AGAGTAC15900.027.31223511
CAGAGTA15950.027.01053210
GCAGAGT16300.026.6419969
GCCAGAG10400.025.6500770
ACTGGTG17000.024.87725670
CACACAG4750.024.65567870
TAAGGCC700.001345304524.6054674
Produced by FastQC (version 0.11.5)