FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940015
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGGGTGTCGTCATAGGA15100.16063573453615101No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA13990.14882741232852667No Hit
CTCCTGCACTGGGAGCAGTTCCAACATCGGGTCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC13990.14882741232852667No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGG13760.146380642862082No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13760.146380642862082No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12550.13350850784295995No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12420.13212555118801295No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11070.11776407823279415No Hit
GTATATTACTGTGCGAGGGCGAGCAGTTGTAGTGGTGGTAGCTGCTACTCATCAAACTGGTTCGACCCCTGGGGC10880.11574283389094854No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG10730.11414711467370202No Hit
GTCCTGGGCCCAGTCTGTGCTGTCACAGCCGCCCTCGGTGTCTGGGGCCCCAGGGCAGAGAATCACCATCTCCTG10680.11361520826795317No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10270.10925357574081264No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10210.10861528805391403No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10170.10818976292931495No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC10030.1067004249932182No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10020.10659404371206843No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9980.10616851858746934No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9830.10457279937022282No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA200.00743506151.61490212
GTATCAA19400.042.9648741
GGTATCA7150.042.3912851
TATCAAC21050.039.3915062
TCAACGC22100.037.5199664
ATCAACG22450.036.9350243
CAACGCA22800.036.368045
TCGTATG1450.035.8616640
AACGCAG23500.035.2847376
CGTATGC1550.033.57319641
CGACTTA450.003879811431.78369967
AGAGTAC26450.031.3527211
CGCAGAG26550.031.1017198
CAGAGTA27150.030.41762410
TCTCGTA1600.030.292438
TATGCCG1750.029.77618443
GCAGAGT28200.029.5275259
CTCGTAT1700.028.53034839
AGGGGGG2153.1195668E-928.3424370
CGATTGT1106.737914E-427.69828470
Produced by FastQC (version 0.11.5)