FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940015
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16530.17584825774056798No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGGGTGTCGTCATAGGA14240.1514869443572709No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13650.14521044876943454No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13150.1398913847119461No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13130.13967862214964655No Hit
CTCCTGCACTGGGAGCAGTTCCAACATCGGGTCAGGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGC12980.13808290293240003No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA12500.13297660143721113No Hit
GTATATTACTGTGCGAGGGCGAGCAGTTGTAGTGGTGGTAGCTGCTACTCATCAAACTGGTTCGACCCCTGGGGC10980.11680664670244624No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGAACCACTCAGG10510.1118067264884071No Hit
GTCCTGGGCCCAGTCTGTGCTGTCACAGCCGCCCTCGGTGTCTGGGGCCCCAGGGCAGAGAATCACCATCTCCTG10250.10904081317851312No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10170.10818976292931495No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9880.10510470577597165No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG9830.10457279937022282No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9720.10340260527757536No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9540.1014877422168795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16950.041.758841
GGTATCA6800.040.423321
TATCAAC18950.037.325752
ATCAACG20000.035.3661463
TCAACGC20100.035.1901974
CAACGCA20500.034.5035555
AACGCAG21100.034.1733326
CGCAGAG22750.031.4130258
ATCGATT450.00439632430.98238647
AGAGTAC23550.030.34752811
CAGAGTA24400.029.29034210
GCAGAGT24850.029.174749
AACCGTT605.5573473E-428.62861316
GCCTAAT1355.7235207E-527.89740270
AGTACTT9550.027.70373213
GCCGTTA756.3424755E-527.48346915
GAGTACT10200.025.93829512
GTACTTT10200.025.93829514
ACGCAGA28700.025.6120387
ATACGGC5500.024.9716846
Produced by FastQC (version 0.11.5)