FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742740
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGAGCTGGAGCCTGAGAAGCGATGAGGGATCCCGTCCCCCAGGGT33880.4561488542423998No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGACAGTAATAGT32840.44214664620190103No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAGTGGCCCATGTCTGA29920.4028327543958855No Hit
CTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACT26380.35517139241188034No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25680.34574682930769857No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21850.2941810054662466No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19490.2624067641435765No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT19000.25580956997064924No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18740.2523090179605246No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC18590.25028946872391417No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18080.2434230013194388No Hit
GCTCTGGGACTGAGCGCTACCTCACCATCTCCAGGCTCCAGTCTGAGGAT17600.2369604437622856No Hit
CACCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTC17470.23521016775722323No Hit
CTCCTGTACAGCCTCTGGATTCATCTTTGGTTCCTATGCCATGAACTGGGCCCGCCAGGCTCCAGTAAAGGGG17470.23521016775722323No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16570.2230928723375609No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16320.21972695694321026No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC15220.20491692920806742No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15000.20195492366103887No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14380.19360745348304925No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14290.192395723941083No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14180.19091472116756875No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCTGGAGATGGTGAGGTAGC14130.1902415380886986No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13920.18741416915744408No Hit
CCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT13590.1829711608369012No Hit
CTCTGGGACTGAGCGCTACCTCACCATCTCCAGGCTCCAGTCTGAGGATG13230.17812424266903626No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGCCAG13000.17502760050623367No Hit
CCTGCACTCTGGCCAGTGGGCACAGCACCTACGCCATCGCGTGGCTTCAG12990.17489296389045966No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12910.17381587096426743No Hit
TCCTCAGACTGGAGCCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGCCTGAGAAGCGATGAGGGATC12500.16829576971753238No Hit
GGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCAC12270.1651991275547298No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT12070.16250639523924928No Hit
GGGCTGAACAGAGAGAAGTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT12060.16237175862347525No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11810.1590058432291246No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11680.15725556722406225No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11620.15644774752941812No Hit
GCCACACCCTGGGGGACGGGATCCCTCATCGCTTCTCAGGCTCCAGCTCTGGGACTGAGCGCTACCTCACCATCT11580.155909201066322No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11420.15375501521393758No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11330.15254328567197137No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11220.15106228289845708No Hit
ATCAATGCCAGTGGCCCATGTCTGACAGTAATAGTCAGCCTCATCCTCAG11180.15052373643536096No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11100.14944664350916875No Hit
GCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAG10840.14594609149904408No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10830.14581145488327005No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10600.14271481272046746No Hit
GACTATTACTGTCAGACATGGGCCACTGGCATTGATTGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT10560.14217626625737137No Hit
GTCTGAGGATGAGGCTGACTATTACTGTCAGACATGGGCCACTGGCATTG10360.13948353394189084No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10220.13759862132105447No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10150.1366561650106363No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10060.13544443546867005No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9970.13423270592670383No Hit
CCCTGGGGGACGGGATCCCTCATCGCTTCTCAGGCTCCAGCTCTGGGACTGAGCGCTACCTCACCATCTCCAGG9960.1340980693109298No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9730.1310014271481272No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9730.1310014271481272No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9680.13032824406925708No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAAT9650.129924334221935No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9610.12938578775883888No Hit
GGGTGTGGCTGCCGTCACTTTTAACTTTCATTAAGTATCGAGGGCCCTTC9440.12709696529068046No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9430.12696232867490642No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9410.12669305544335838No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9290.12507741605407005No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9160.12332714004900773No Hit
GAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTG9130.12292323020168565No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9130.12292323020168565No Hit
CCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGGCCAGTGGGCACAGC9090.12238468373858954No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCAATCAATGC9010.12130759081239735No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8840.1190187683442389No Hit
GTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCA8800.1184802218811428No Hit
CTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGC8730.11753776557072461No Hit
GCACCTACGCCATCGCGTGGCTTCAGAAGCAGCCAGGGAAGGGCCCTCGA8660.11659530926030642No Hit
GTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCA8550.11511430648679215No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC8540.11497966987101813No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8510.11457576002369604No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG8460.11390257694482592No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA8430.11349866709750382No Hit
TCCTCACCCTCCTCCTCCACTGCACAGGGTCTCTCTCCCAGGTTGTGCTGACTCAATCGCCCTCTGCCTCTGCCT8340.11228693755553759No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8320.11201766432398955No Hit
ATATTACGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAGACAATT8270.11134448124511943No Hit
CTACCTCACCATCTCCAGGCTCCAGTCTGAGGATGAGGCTGACTATTACT8190.11026738831892721No Hit
GTATCAACGCAGAGTACGGGAGAGCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTC8190.11026738831892721No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG8160.10986347847160513No Hit
CTCCAGGCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGACATGGGCCACTGGCATTGATTGGGTGTTCGG8040.10824783908231683No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8010.10784392923499475No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7970.10730538277189865No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7900.10636292646148046No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7770.10461265045641813No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT7770.10461265045641813No Hit
GCACAGCACCTACGCCATCGCGTGGCTTCAGAAGCAGCCAGGGAAGGGCC7760.10447801384064409No Hit
GTGCTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCAAGCTCACCTGCACTCTGGCCAGTGGG7710.10380483076177396No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7690.10353555753022592No Hit
GGGAAGGGCCCTCGATACTTAATGAAAGTTAAAAGTGACGGCAGCCACACCCTGGGGGACGGGATCCCTCATCG7560.10178528152516358No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCTGGAGATGGTGAGGTAGCGCTCAGTCCCAGAGCTGGAGC7500.10097746183051944No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7460.10043891536742333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT1200.048.907971
GTATCAA8500.041.427931
GGTATCA3400.040.6156161
TGGTATC1450.040.4564862
TATGTGC350.001346167139.4365732
TATCAAC9600.036.663692
ACTTTCA12950.034.5614570
TTCGGGG6850.032.669470
ATCAACG10950.032.143513
TCAACGC10950.032.143514
AACGCAG11100.031.3982626
TTAGGCA553.2520547E-431.374
CAACGCA11150.031.2574655
CCTTTAA450.004375841531.01112757
TAGCCTT450.004617062430.672895
GGGCAGT2150.030.508941
TACGGGT802.7555507E-630.1936264
GAGTACT2900.029.74541312
CGCAGAG12200.028.2825248
AGAGTAC12250.027.88541411
Produced by FastQC (version 0.11.5)