FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857134
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42540.4963051284863277No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30900.36050372520516044No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26590.3102198722720135No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25380.2961030597316172No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25290.29505304888150513No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23680.2762695214517217No Hit
GAGCTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCAT22420.26156936955015203No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22410.26145270167791734No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22120.2580693333831116No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21620.25223593977137765No Hit
ATGCTGCATCCGGCTTGCAGAGTGGGGTCCCATCAAGATTCAGTGGCAGAGGATCTGGGACAGATTTCACTCTCA19070.2224856323515343No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18500.21583556363415757No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18330.21385220980616798No Hit
GTACTGTAGCTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT18260.21303553470052522No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17960.20953549853348485No Hit
GTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTTGGCCGAACGTC17950.20941883066125017No Hit
CTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTT17950.20941883066125017No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16790.1958853574820273No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16720.19506868237638456No Hit
GATCTATGCTGCATCCGGCTTGCAGAGTGGGGTCCCATCAAGATTCAGTGGCAGAGGATCTGGGACAGATTTCA16580.19343533216509903No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16450.1919186498260482No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16360.1908686389759361No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15800.18433523813079403No Hit
ACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGA15760.1838685666418553No Hit
CTGTAGCTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC15610.1821185485583351No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15540.18130187345269236No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC15510.18095186983598832No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15350.17908518388023342No Hit
CTCCTGATCTATGCTGCATCCGGCTTGCAGAGTGGGGTCCCATCAAGATT15120.1764018228188358No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14780.17243511516285667No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14650.17091843282380584No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14220.1659017143177146No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13760.16053499219491935No Hit
GAGTGGGGTCCCATCAAGATTCAGTGGCAGAGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAAC13680.1596016492170419No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13330.15551827368882812No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT13240.15446826283871598No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12900.1505015551827369No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAGTTTAAAT12900.1505015551827369No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCTCTGCCA12770.14898487284368606No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT12650.14758485837686988No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGCTACAGTACC12480.14560150454888035No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGCTACAGTACCCCTCGGACGTT12480.14560150454888035No Hit
GGACCAAGGTGGACATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12320.14373481859312545No Hit
CTCCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGTTATTTAAACTGGTATCAACAGAAACCAGGGAAAGCCC12320.14373481859312545No Hit
GGGTACTGTAGCTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT12010.14011811455385037No Hit
ACTTACTACTGTCAACAGAGCTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAA11970.13965144306491167No Hit
GTCTCCGTCCTCCCTGTCTGCTTCTGTGGGAGACAGAGTCTCCATCACTT11930.13918477157597295No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAGTT11860.13836809647033022No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11790.13755142136468743No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11730.13685141413127935No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11670.13615140689787128No Hit
GTGGGGTCCCATCAAGATTCAGTGGCAGAGGATCTGGGACAGATTTCACT11640.13580140328116724No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11220.13090135264731068No Hit
CCTTGGTCCCTTGGCCGAACGTCCGAGGGGTACTGTAGCTCTGTTGACAG11090.12938467030825987No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10900.12716798073580093No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10900.12716798073580093No Hit
ATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAGTTTAAATAACTGCTAATGCT10900.12716798073580093No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10890.12705131286356625No Hit
GTCTGCTTCTGTGGGAGACAGAGTCTCCATCACTTGCCGGGCAAGTCAGA10760.12553463052451544No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATGTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGGTA10650.12425128392993395No Hit
ACTGTCAACAGAGCTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAACGAACTGTGGCTG10580.12343460882429118No Hit
GAGTGAAATCTGTCCCAGATCCTCTGCCACTGAATCTTGATGGGACCCCA10490.12238459797417905No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10330.1205179120184242No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10150.11841789031819996No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10130.1181845545737306No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCGTCCTCC10120.1180678867014959No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10000.11666787223467975No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9940.11596786500127168No Hit
GTCCCAGATCCTCTGCCACTGAATCTTGATGGGACCCCACTCTGCAAGCC9910.11561786138456764No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGATCCTCTGCCACTGAATCTTG9890.11538452564009828No Hit
GTGGGAGACAGAGTCTCCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGTTATTTAAACTGGTATCAACAGAAA9820.11456785053445553No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC9720.11340117181210872No Hit
CTGCAAGCCGGATGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAGTTTAAATAA9710.11328450393987405No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGTTGATACCAGTTTAAATAACTGCTA9650.11258449670646596No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCTCTGCCACTGAATCT9570.11165115372858853No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGCTACAGTACCCCTCGGACGTTCGGCCAAGGGA9490.11071781075071109No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC9300.10850112117825217No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9250.10791778181707878No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9200.10733444245590539No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9160.10686777096696666No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9100.10616776373355857No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8990.1048844171389771No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8850.10325106692769159No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT8800.10266772756651818No Hit
GTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCT8770.10231772394981414No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTACG200.006883385652.63064254
ATACGGT200.007514324551.47623431
GTGGTAT2000.040.98151
CAACGCA15950.033.1722455
GTGTATA1650.033.1163641
TCAACGC16050.032.965574
ATCAACG16100.032.6511653
AACGCAG16500.032.4802676
TGTTAGA9400.031.29525270
AGAGTAC17900.029.58612611
CGCAGAG18000.029.4183248
ACGCAGA18400.029.1382247
TGTATAA2150.025.403022
CAGAGTA20900.025.33931410
CAGTTCG15300.025.20892770
TATAAGA3150.024.9241962
AGTACGG16050.023.41677513
AGTACTT6150.023.333713
GAGTACT6500.023.12856912
GAGTACG16550.022.91576812
Produced by FastQC (version 0.11.5)