FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences996137
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15460.15519953580682175No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12910.12960064730052193No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10310.10349981980390247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4950.038.1089321
GTATCAA29450.036.5690461
CTAGTAC400.002675286534.289433
TATCAAC32200.033.4391982
ATCAACG33300.032.1270333
TCAACGC33700.031.847454
CAACGCA34250.031.3360355
AACGCAG34700.031.0284756
ACGCAGA36100.029.8311447
AGAGTAC36450.029.08884611
CGCAGAG37400.028.5291548
CATATCG500.00705687628.1232658
CAGAGTA37950.028.02950710
GCAGAGT38200.027.7548489
TCCGAGA3009.640644E-1125.43712670
GAGTACG35250.025.11459412
AGTACGG35150.025.0884213
GCTTGAT2400.024.2944431
TGTGTCG3452.1827873E-1123.9625170
TACGGGG17400.023.86291515
Produced by FastQC (version 0.11.5)