FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005754996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569990
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCACCAGTATGGTGACTCACCGCGGACGTTCGGCCAAGGGA14910.2615835365532729No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13430.23561816873980246No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13010.22824961841435817No Hit
GTATATCATCACTAGCCAGTTAGCCTGGTACCAGCAGAAACCTGGCCGGG11980.21017912594957808No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA8830.15491499850874577No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGACCGCTGATGGTGAGAGTGAAGTCTCTTCCAGACCCACTGCCA8690.152458815066931No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT8380.14702012316005544No Hit
GTATGGTGACTCACCGCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT8300.1456165897647327No Hit
GTGTATTACTGTCACCAGTATGGTGACTCACCGCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA8290.14544114809031736No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7720.13544097264864297No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7300.12807242232319865No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC6420.11263355497464868No Hit
TCTATGGTGCGTCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGAAGAGACTTCACT6240.10947560483517255No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5970.10473867962595834No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5700.10000175441674415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)