FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005754999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005754999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072317
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT26420.2463823664084408No Hit
GGTTAGGAGACTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTACTGGAGCCGTATAAAACACT22150.20656205207974881No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21980.2049766999870374No Hit
GAGTAGTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT19410.18100990658545935No Hit
ATACTACTCCGTTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGA18520.17271012210008796No Hit
AGCCAGGACAGTCTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAA17900.16692824976196405No Hit
GTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC17750.16552940968015986No Hit
ATATCATACTACTCCGTTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGAACTGTGGCTGCACCAT17220.1605868413911185No Hit
GTTTTATACGGCTCCAGTAGTAAGAACTACTTAGCTTGGTACCAGCAGAA15830.14762425663306653No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15750.14687820858943762No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15130.14109633625131374No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14900.13895144812588067No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14740.1374593520386229No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14110.1315842236950454No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13550.12636188738964318No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13200.12309792719876678No Hit
GTAATACACGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATATA13140.12253839116604512No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTCTGGTCCCAGGGCCGAAAGTG12800.11936768698062235No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12770.11908791896426149No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12700.11843512692608622No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12110.11293302260432317No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTACTGGAGCCG12100.11283976659886956No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11980.11172069453342622No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11400.10631184621711677No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATCATACTACTCCGTTCACTTT11250.1049130061353126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7250.040.9527851
GTATCAA17450.039.5692251
TGCGTTA700.002381875435.1091170
TATCAAC19900.034.4935652
TGGTATC3350.033.9787372
TCAACGC20300.033.813894
CAACGCA20500.033.6522565
ATCAACG20650.033.407813
AACGCAG20900.033.0081986
GTGGTAT3700.029.8587251
CGCAGAG23450.029.5659148
AGAGTAC23050.029.480411
CAGAGTA23600.028.79335610
ACGCAGA24050.028.5414547
GTACGGT500.00774173527.5948511
GCAGAGT25300.027.5403169
GCAACCG1200.001067445325.60039570
GTATAAG2400.024.4546071
TCGTATG1001.5095813E-524.25974740
TGTTAGA4150.023.68807670
Produced by FastQC (version 0.11.5)