FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072317
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCT26520.2473149264629769No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT22710.21178438838515107No Hit
GGTTAGGAGACTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTACTGGAGCCGTATAAAACACT19120.17830548242730462No Hit
GTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAATCTGTC17880.16674173775105683No Hit
GAGTAGTATGATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAAAT17880.16674173775105683No Hit
ATACTACTCCGTTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGA17070.15918800130931432No Hit
GTTTTATACGGCTCCAGTAGTAAGAACTACTTAGCTTGGTACCAGCAGAA16990.15844195326568541No Hit
ATATCATACTACTCCGTTCACTTTCGGCCCTGGGACCAGAGTGGATATCAAACGAACTGTGGCTGCACCAT16650.15527124908026266No Hit
AGCCAGGACAGTCTCCTAACCTGCTCATTTACTGGGCATCTACCCGGGAA16310.15210054489483987No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14770.13773912005498373No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14650.13662004798954042No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14250.13288980777139597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13850.1291595675532515No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13060.12179234312241621No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12690.11834187092063261No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12510.11666326282246761No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12400.11563744676247789No Hit
GTAATACACGGCCGTGTCCTCAGCTCTCAGACTGTTCATTTGCAGATATA12080.11265325458796234No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTCTGGTCCCAGGGCCGAAAGTG11940.11134767051161178No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11910.11106790249525093No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11260.10500626214076621No Hit
CACCATCACCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATCATACTACTCCGTTCACTTT11070.10323439803714761No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC10870.10136927792807537No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTACTACTGGAGCCG10800.1007164858899001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17050.038.800871
TCGATTG400.00261136734.45919413
TATCAAC19200.033.7176932
GTGGTAT3950.033.1475221
ATCAACG19650.032.9455343
TCAACGC19950.032.6227234
CAACGCA20600.031.7605235
AACGCAG21150.031.0974126
GGTATCA7650.030.6275391
TGGTATC4200.029.5157932
CGCAGAG23100.028.1886238
AGAGTAC22650.027.84120611
CGATTGT500.00777948327.56735614
CAGAGTA23300.027.21241210
ACGCAGA24200.027.0446457
GCAGAGT25000.026.0511519
TTAACGG1101.1286375E-625.151635
ACTTGCA7600.024.0110970
TACGGGT1554.1394742E-822.230715
AGTACGG24500.021.80071413
Produced by FastQC (version 0.11.5)