FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272589
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATTCCACACGATCACTTCGATCACTACCCCT45780.35973908308181196No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATTCCACACGATCACTTCGATCACTACCCCTCTCCCAGAC44530.3499165873663846No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37130.29176741273105455No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG34240.2690578026369865No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32630.256406428155516No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29490.2317323189183625No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG28420.22332426258595667No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTC27040.21248022731612481No Hit
GTGTTGGTCGTCTGGGATAACAACCGGCCCTCAGGGAACCCTGAGGGATT26920.21153726772744383No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26350.20705820968120892No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25790.20265773160069747No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25640.2014790321148462No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25470.20014317269754808No Hit
CCCCTGTGTTGGTCGTCTGGGATAACAACCGGCCCTCAGGGAACCCTGAG25360.19927879307459045No Hit
GCTTAGTCCCTCCGCCGAATTCCACACGATCACTTCGATCACTACCCCTCTCCCAGACCTGACAATAATAGTCGG24780.19472115506263216No Hit
ACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATTCCACACGATCACTTC23620.18560587903871556No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21790.1712257453113299No Hit
GTAATATATTGCCGTGTCCTCGACTCTGAGGCTGTTCATCTCCAAAAACA21290.16729674702515895No Hit
ATATATTACTGTGCGAGAGAGAGGGGGGATTTTTGTGGTAGTGACTGCTA21050.16541082784779687No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20290.15943875045281705No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20050.157552831275455No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19630.1542524727150714No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19430.15268087340060302No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACT18940.1488304550801555No Hit
CTCCCAGACCTGACAATAATAGTCGGCCTCATCCCCGACTTCGACCCTGC18520.1455300965197719No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAC18380.14442997699964402No Hit
GGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG18350.14419423710247378No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18330.14403707717102693No Hit
CTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT18230.14325127751379274No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18120.14238689789083514No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17880.14050097871347308No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGTCGGGGATGAGGCCGACTAT17620.1384578996046642No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGC17540.13782925987887684No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTGGGATAACAACCGGCCCTCAGGGAACCCTGAGGGATTCTCTGGCTCCAA17480.13735778008453634No Hit
GCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA17380.13657198042730212No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17300.1359433407015148No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17160.13484322118138692No Hit
CTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTAC16520.1298141033750881No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16240.12761386433483238No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCAC16200.1272995444719387No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15630.12282048642570383No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG15490.12172036690557596No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15190.11936296793387338No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15170.11920580800242654No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCCCTCAGGGTTCCCTGAGGGCCGGTTGTTATCCCAGACGA14950.11747704875651134No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCACGATTACCTGTGGGGGAGAGAA14940.11739846879078791No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTTGGTCGTCTGGGATAAC14820.11645550920210687No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTA14790.11621976930493663No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA14780.11614118933921322No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14460.1136266304360638No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGA14440.11346947050461696No Hit
CTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG14400.1131551506417233No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14340.11268367084738277No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13930.1094618922527226No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13880.10906899242410549No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13680.10749739310963713No Hit
CGATTACCTGTGGGGGAGAGAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC13620.10702591331529661No Hit
AGCCTGGGGAGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC13420.10545431400082823No Hit
CTATAGCATGAACTGGGTCCGCCAGGCTCCGGGGAAGGGGCTGGAGTGGGTCGCATCCATAAGTAGTAGTAGTCG13360.10498283420648771No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13040.10246827530333831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7800.047.625931
GTATCAA17600.037.7188951
TATCAAC20250.032.9397542
TCAACGC20800.032.0687524
ATCAACG21200.031.4636783
CAACGCA21250.031.3896485
AACGCAG21450.031.2572656
TCGTATG450.00455137530.76452440
GTGGTAT2500.030.2689251
TTCGGGG10850.028.41516570
CCGTTAA854.545882E-628.31651716
CTCGTAT500.007639427727.6705639
TACCTAG656.934004E-427.56622367
CCGTCTA500.00783892227.52477827
AGAGTAC24150.027.33661811
ACGCAGA24600.027.254817
CGCAGAG24550.027.1702678
CAGAGTA24950.026.46009610
TGGTATC2900.026.0836492
GCAGAGT26350.025.053269
Produced by FastQC (version 0.11.5)