FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272589
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATTCCACACGATCACTTCGATCACTACCCCT45410.35683162435004545No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATTCCACACGATCACTTCGATCACTACCCCTCTCCCAGAC43320.3404084115138509No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36880.2898029135879691No Hit
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG31140.2446980132627266No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29480.23165373895263908No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27680.21750934512242368No Hit
GTGTTGGTCGTCTGGGATAACAACCGGCCCTCAGGGAACCCTGAGGGATT26530.2084726490642305No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26480.2080797492356134No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25930.2037578511208253No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG24970.19621417441137712No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTC24860.1953497947884195No Hit
GCTTAGTCCCTCCGCCGAATTCCACACGATCACTTCGATCACTACCCCTCTCCCAGACCTGACAATAATAGTCGG24780.19472115506263216No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24420.1918922762965891No Hit
CCCCTGTGTTGGTCGTCTGGGATAACAACCGGCCCTCAGGGAACCCTGAG23880.18764895814752444No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23630.18568445900443897No Hit
ACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAATTCCACACGATCACTTC22140.17397604411164957No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21610.16981130592830834No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20260.1592030105556468No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19140.15040205439462387No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACT18780.1475731756285808No Hit
ATATATTACTGTGCGAGAGAGAGGGGGGATTTTTGTGGTAGTGACTGCTA18760.14741601569713395No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18740.14725885576568712No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGC18530.1456086764854953No Hit
GTAATATATTGCCGTGTCCTCGACTCTGAGGCTGTTCATCTCCAAAAACA18520.1455300965197719No Hit
CTCCCAGACCTGACAATAATAGTCGGCCTCATCCCCGACTTCGACCCTGC18370.1443513970339206No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18240.14332985747951618No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAC17790.1397937590219623No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17640.13861505953611103No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17150.1347646412156635No Hit
GGACTAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG17130.13460748128421668No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCCCTCAGGGTTCCCTGAGGGCCGGTTGTTATCCCAGACGA16660.13091422289521595No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16510.12973552340936467No Hit
GCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA16330.12832108402634315No Hit
GGCCAGGCCCCTGTGTTGGTCGTCTGGGATAACAACCGGCCCTCAGGGAACCCTGAGGGATTCTCTGGCTCCAA16160.12698522460904502No Hit
CTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGT16090.1264351648489811No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15810.12423492580872536No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15380.12085598728261834No Hit
CTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCACGATTAC15250.1198344477282139No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGA15200.1194415478995968No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14840.11661266913355371No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCAC14760.11598402940776638No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14640.11504106981908534No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGTCGGGGATGAGGCCGACTAT14630.11496248985336191No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG13940.10954047221844601No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTA13910.10930473232127576No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13750.10804745286970105No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13670.10741881314391372No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGTTGGTCGTCTGGGATAAC13510.10616153369233901No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCACGATTACCTGTGGGGGAGAGAA13510.10616153369233901No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13490.10600437376089217No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT13390.10521857410365797No Hit
CTATAGCATGAACTGGGTCCGCCAGGCTCCGGGGAAGGGGCTGGAGTGGGTCGCATCCATAAGTAGTAGTAGTCG13380.10513999413793455No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA13240.1040398746178067No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13220.10388271468635984No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13130.1031754949948491No Hit
CTCACTGCACAGACTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG12980.10199679550899779No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12890.10128957581748703No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12860.10105383592031678No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12740.10011087633163575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8400.045.755171
GTATCAA18350.036.466991
TTAACGC400.002364831535.1634759
TGGTATC3000.034.309632
GTGGTAT3450.033.8190351
TATCAAC21050.031.620282
ATCAACG22050.029.7194563
TCAACGC22550.029.0604864
CAACGCA22650.028.9321865
TTCGGGG8900.028.85493570
AACGCAG23250.028.1855476
GCCTAAT950.00855754627.03251670
CTTAACG657.7916187E-427.02162258
ATTAGAG1850.025.9640463
CGCAGAG25600.025.2070318
GTATACC1101.2078199E-624.9573651
AGAGTAC26400.024.57706511
CAGAGTA26400.024.57706510
TCTGGAG13200.024.31902770
CGCAACT8150.024.01633652
Produced by FastQC (version 0.11.5)