FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559539
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15450.043.2515141
GTATCAA39700.039.879221
ACTCGAC2700.036.8388227
TATCAAC46200.034.2531282
ATCAACG47500.033.0993353
TCAACGC47700.033.0323644
TCGTATG2750.032.71093840
CAACGCA49400.031.8956225
AACGCAG51050.031.1331046
ATACCGC704.0453087E-529.3598217
CGTATGC3450.029.12491441
TATGCCG3450.028.18825543
CTCGTAT3200.028.08095439
GTGGTAT6650.027.8270591
CGACGTC3500.027.47384830
ACGCAGA57750.027.4618137
TCGACGT3500.027.4606129
AGAGTAC58900.026.69386911
CGCAGAG59050.026.6832168
GACGTCA3550.026.13294631
Produced by FastQC (version 0.11.5)