FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559539
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30880.19800723162421716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39450.040.8914261
GGTATCA15750.039.626771
TATCAAC43400.036.843592
TCAACGC45900.034.6140254
ATCAACG46500.034.1673933
CAACGCA47650.033.128135
AACGCAG48300.032.8940736
GTGGTAT7300.032.2362371
TGGTATC6900.030.6352522
ACGCAGA52900.030.1068577
CGCAGAG54350.029.3092788
AGAGTAC54750.029.29238911
CAGAGTA56550.028.29966410
GCCTAAT3300.027.78326270
GCAGAGT59350.027.0211759
AGTACGG35850.026.07969313
GAGTACG36000.026.06581312
ACGGGAT3450.024.72725316
TACGGGG21700.023.74728815
GTACGGG40100.023.23054114
Produced by FastQC (version 0.11.5)