FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956618
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20610.215446500065857No Hit
CTCCTGCACTGGGAGCAGGTCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAGCAGCCTCCTGGAACAGC19750.20645649569629673No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGG16850.17614136468266328No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT15660.16370170747362062No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG15590.1629699629319122No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15430.1612974039794359No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGGC14630.15293460921705423No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC14300.14948495637757184No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG14130.14770786249056572No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13500.1411221616151902No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12930.13516367034699328No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGGCTGCTGTCATAGGA12520.13087773803127267No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12350.12910064414426659No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11650.12178319872718263No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11490.12011063977470632No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11430.11948343016752769No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC11410.11927436029846815No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGGTGGGATGGATCAACCCTGATAGTGGTGG11050.1155111026553964No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11010.11509296291727733No Hit
GTCCTATGACAGCAGCCTGAGTGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGC10790.11279319435762236No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC10510.10986621619078879No Hit
GGTTATGATGTACATTGGTACCAGCAGCCTCCTGGAACAGCCCCCAAACTCCTCATCTATGTTAACAACAATCGG9790.10233970090464531No Hit
GGGTCACCCTGACCAGGGACACGTCAATCAGCACTGCCTACATGGAACTG9600.10035353714857968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)