FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956618
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGGTCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAGCAGCCTCCTGGAACAGC18580.1942259083563136No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16000.1672558952476328No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC14930.1560706572529474No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT14840.15512984284217943No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG14830.15502530790764965No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC14190.14833507209774432No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13930.14561716379997033No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGG13460.1407040218770711No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC13150.13746343890664822No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12710.1328639017873383No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG12160.12711448038820092No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12120.12669634065008184No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGGC11830.1236648275487185No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG11700.1223058733998315No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11150.11655645200069412No Hit
GTCCTATGACAGCAGCCTGAGTGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGC11110.11613831226257502No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC11060.11561563758992618No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGGCTGCTGTCATAGGA10890.11383854370292008No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC10790.11279319435762236No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10510.10986621619078879No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10250.10714830789301476No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10210.1067301681548957No Hit
CTATATGCACTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGGTGGGATGGATCAACCCTGATAGTGGTGG10030.10484853933335982No Hit
GGTTATGATGTACATTGGTACCAGCAGCCTCCTGGAACAGCCCCCAAACTCCTCATCTATGTTAACAACAATCGG9870.10317598038088349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16450.039.5252531
TATCAAC17850.036.2249342
GGTATCA7300.035.344121
GTGGTAT3500.034.4016081
TCAACGC19200.033.6778684
TGGTATC3500.033.411722
CAACGCA19500.033.336135
ATCAACG19550.033.074943
AACGCAG20100.032.5121356
CGTATTA450.00468014630.58880615
AGAGTAC21400.030.2330411
ACGCAGA21950.029.4616247
CAGAGTA22000.029.408510
CGCAGAG22800.028.6695088
GTACTAA605.512888E-428.6680071
GCAGAGT23350.028.1474349
TATAAGA1551.5788828E-924.4089412
GAGTACT6650.024.32282312
GTATAAG2450.023.8705041
AGTACGG20400.023.61759213
Produced by FastQC (version 0.11.5)