FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023441
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGGTCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAGCAGCCTCCTGGAACAGC20850.20372449413302768No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT20150.19688482286717066No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19990.19532146943497475No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGG19300.18857950775862997No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA18260.17841771044935661No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG16710.16327272407495888No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC16030.15662847198812635No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGGC15410.15057047743836724No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15170.1482254472900734No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14950.14607583632080404No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG14340.14011555136055717No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12820.12526369375469615No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGGCTGCTGTCATAGGA12600.12311408278542682No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC12370.12086676222664522No Hit
GTCCTATGACAGCAGCCTGAGTGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGC12220.11940111838396157No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC12060.11783776495176566No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11700.11432021972932488No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11680.11412480055030041No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10480.10239964980883118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19350.041.0829961
GGTATCA7850.039.0167851
AGGGGGG3050.038.32127870
TATCAAC21900.036.2745322
ATCAACG22700.034.3901373
TCAACGC22950.034.015524
TCGTATG2200.033.04816440
CAACGCA23700.032.939085
GTGGTAT3200.032.2628251
AACGCAG24300.032.1257676
TATACAC2300.031.3996933
TATCTCG2450.029.61036536
TTATACA2450.029.4772622
AGAGTAC26750.029.05484811
ACGCAGA26950.028.9654187
CGCAGAG26900.028.8914188
TCTCGTA2550.028.48412338
CGTATGC2450.028.28085141
CAGAGTA28000.027.75775510
TGGTATC3800.027.1501082
Produced by FastQC (version 0.11.5)