FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023441
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCAGGTCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAGCAGCCTCCTGGAACAGC20420.1995229817840012No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT18830.1839871570515545No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17040.1664971405288629No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17000.16610630217081396No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC16090.1572147295251998No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15780.15418573225032026No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGG15370.15017963908031826No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14550.1421674527403143No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG13790.13474152393738378No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTG13750.1343506855793348No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12900.12604537047079412No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12810.12516598416518393No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGGC12770.12477514580713496No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG12740.12448201703859822No Hit
GTCCTATGACAGCAGCCTGAGTGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGC12090.1181308937203024No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC11720.11451563890834937No Hit
GTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC11540.11275686629712901No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11260.1100209977907862No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAACCACTCAGGCTGCTGTCATAGGA11170.10914161148517598No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC10980.10728512928444336No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG10720.10474467995712504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19850.039.0889781
GGTATCA8150.037.0707551
TATCAAC20900.036.937432
ATCAACG21600.035.7386363
TCAACGC22300.034.770654
CAACGCA22500.034.6140635
CGCGTAT501.917999E-434.32418412
AACGCAG23500.033.2887466
ACGCAGA25350.031.1361737
CGCAGAG25900.030.4794468
AGAGTAC25050.030.28201511
GTGGTAT3200.030.0410061
CAGAGTA26250.029.2899710
GCCTAAT1304.1716805E-529.222870
GCAGAGT27650.028.1793529
TACGGGT1705.456968E-1226.24662415
AGTACGG21200.025.7431413
GAGTACG21250.025.68256612
TGGTATC3700.025.037592
CGTATTC700.001374045724.51727514
Produced by FastQC (version 0.11.5)