FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1298886
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41400.31873466955529584No Hit
GTATTACTGTCAGCAGTTTGATAGCTCACCGTACACTTTTGGCCAGGGGA29550.22750264457388872No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27620.21264375780476502No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA19000.14627919617272034No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17050.13126633130236218No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16150.12433731674681225No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15950.12279753573446785No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT15220.1171773350394107No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14920.11486766352089406No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14890.11463669636904239No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14620.11255799200237743No Hit
GTGTATTACTGTCAGCAGTTTGATAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14550.11201906864805686No Hit
CATCTATGCTGCGTCCAGCAGGGCCACTGGCATCCCTGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA13150.10124060156164591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)