FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727030
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT11780.162029077204517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA253.0446263E-669.1843538
ATATCGT309.04988E-657.5776937
GTGGTAT4350.042.5697251
TGGTATC4400.042.0715072
AGGGGGG3550.040.36874870
GTATCAA26200.039.265971
TCGTATG2450.038.18824840
GGTATCA11150.037.5215951
CTCGTAT2000.036.3571139
ATCGTAT501.8213484E-434.62581639
CTTATAC2700.034.292281
CGTATGC2750.034.05540541
TATCAAC30450.033.7738762
TATACAC2950.033.6994633
ATCAACG32600.031.756773
CAACGCA32600.031.546465
TTATACA3050.031.470612
TCAACGC32700.031.449994
AATATCG553.241995E-431.38643336
AACGCAG32850.031.306386
Produced by FastQC (version 0.11.5)