FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727030
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25250.3473034125139265No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAA150.002418063668.5431110
GTGGTAT5100.044.3422741
TGGTATC5650.040.009252
GTATCAA28700.039.7563551
TATCAAC34050.033.1941642
AACGCAG33800.033.135696
TCAACGC34400.032.8564344
GGTATCA11500.032.7747231
CAACGCA34550.032.614665
ATCAACG34700.032.5723763
AGAGTAC34900.031.03098111
CAGAGTA35350.031.02375810
ACGCAGA36350.030.7211787
CGCAGAG36750.030.5774258
GCAGAGT37250.029.9933649
TATATAC500.00801202627.4002763
AGTACGG35800.025.84726313
GAGTACG36700.025.21340812
TACGGGG15300.022.62370915
GTACGGG39600.021.72262814
Produced by FastQC (version 0.11.5)