FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966015
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18800.1946139552698457No Hit
GTCTCTGGTGTCTCCATCAGTAGGAGTAGTTACTACTGGGGCTGGATCCG15820.16376557299834887No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC15750.16304094656915266No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15460.16003892279105397No Hit
GTAATAAAGAGCCGTGTCTGCGGCGGTCATAGAGTTCAGGCTCAGGGAGA14430.14937656247573794No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT14160.14658157482026676No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA14000.1449252858392468No Hit
CTTTATTACTGTGCGACACATAGCCAACTGGGAATTAACTACTGGGGCCA13950.14440769553267807No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13720.14202678012246187No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGTAGGAGTAGTTACTACTGGG13220.13685087705677448No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12860.13312422684947955No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12670.13115738368451835No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12370.12805184184510593No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11740.12153020398233982No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11460.11863169826555489No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG11410.11811410795898615No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11110.11500856611957372No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11060.11449097581300498No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11010.11397338550643624No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10820.11200654234147503No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10620.10993618111520007No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGCTACGTAGCCTGGTACCAACAAAAACCTGGCCAGGCTCCCACGCT9970.10320750712980648No Hit
GAGTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG9890.10237936263929649No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT9860.10206880845535524No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9800.10144770008747275No Hit
TCTATGATGCGTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT9760.10103362784221778No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9690.10030900141302153No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)