FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966015
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18930.1959596900669244No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16690.17277164433264494No Hit
GTAATAAAGAGCCGTGTCTGCGGCGGTCATAGAGTTCAGGCTCAGGGAGA14660.15175747788595415No Hit
GTCTCTGGTGTCTCCATCAGTAGGAGTAGTTACTACTGGGGCTGGATCCG14630.1514469237020129No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14240.14740971931077676No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT14180.14678861094289425No Hit
CTTTATTACTGTGCGACACATAGCCAACTGGGAATTAACTACTGGGGCCA13160.136229768688892No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT12920.13374533521736204No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12280.12712017929328218No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12180.12608499868014472No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGTAGGAGTAGTTACTACTGGG12100.12525685418963473No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11880.12297945684073229No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11130.1152156022422012No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10890.11273116877067126No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10700.11076432560571006No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10490.10859044631812136No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10490.10859044631812136No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10370.10734822958235637No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10040.1039321335590027No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGTAG9790.10134418202615901No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9770.10113714590353153No Hit
GAGTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGG9700.10041251947433527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15850.041.7004131
GGTATCA6400.040.8791661
TATCAAC18350.035.6199762
TCAACGC19100.034.581514
GACGTAT400.002567974634.57678635
ATCAACG19200.034.2222253
AACGCAG19800.033.7064176
CAACGCA19700.033.5282675
TACGGAT5950.032.42477870
CGCAGAG21900.030.6472458
ACGCAGA22200.030.0687087
AGAGTAC21700.029.82404311
GTGGTAT3150.029.5067651
CAGAGTA22200.029.1523310
GCAGAGT22950.028.6496339
TATTAAC951.0741222E-525.3483982
TGGTATC3400.025.2951452
AGTACGG22150.023.93400613
CTACGGA5550.023.7931869
GAGTACG22550.023.35679812
Produced by FastQC (version 0.11.5)