FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122490
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGACACGGCCAC62120.5534125025612701No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53790.4792024873272813No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCGCTGTACTGACTCAGC51050.4547924703115395No Hit
CCATTACGTGTGGGGAAAGGTACATTGGCAGTAAAAGTGTCCACTGGTACCAGCAGAAGCCAGGCCAGGCCC49610.4419638482302738No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC47490.42307726572174364No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46620.4153266398809789No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG45660.4067742251601351No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGGATGAAGA43960.3916293240919741No Hit
CCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGACACG42030.37443540699694433No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41480.3695355860631275No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC41240.36739748238291653No Hit
CCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCT38370.34182932587372716No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38100.33942395923348984No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG37340.33265329757948847No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36610.3261498988855135No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34050.30334345962992987No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG33830.30138353125640316No Hit
GTAATAGTCTTCATCCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT33540.2987999893094816No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT32230.28712950672166343No Hit
GACTATTACCATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCCTCGACCCTGGTGATGGTCAGGGTGG32080.28579319192153163No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30100.2681538365597912No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT29440.262274051439211No Hit
CCACAGGATGAAGACTATTACCATCCCACACCTGACAATAATAGTCGGCC28650.2552361268251833No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACACTTTTACTG28330.2523853219182354No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG28040.24980177997131375No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27620.2460600985309446No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGA27590.24579283557091824No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27220.24249659239725965No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27040.24089301463710144No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26690.23777494677012712No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26600.236973157890048No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26550.2365277196233374No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCGCTGTACTGACT25980.23144972338283637No Hit
GTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG25490.22708442836907233No Hit
CGGCCACCCTGACCATCACCAGGGTCGAGGCCGGGGATGAGGCCGACTAT25350.2258372012222826No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25030.22298639631533465No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATGGA24870.22156099386186068No Hit
GGCCAGGCCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGG24860.22147190620851856No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGGATGAAGAC24620.2193338025283076No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24570.218888364261597No Hit
ACCCTGGTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAA24440.21773022476814938No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23860.21256314087430622No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT23770.2117613519942271No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23730.21140500138085863No Hit
CACCAGGGTCGAGGCCGGGGATGAGGCCGACTATTATTGTCAGGTGTGGG23650.21069230015412166No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23130.20605974218033124No Hit
ATCCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC22870.203743463193436No Hit
TGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC22460.20009086940640897No Hit
GTGTCAGTGGCCCCAGGAGACACGGCCACCATTACGTGTGGGGAAAGGTA22000.1959928373526713No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21900.19510196081925005No Hit
GGCTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGC21070.18770768559185383No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA20730.18467870537822162No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20600.18352056588477403No Hit
CTCACTGCACAGGCTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGACACGG20580.1833423905780898No Hit
GGATGAAGACTATTACCATCCCACACCTGACAATAATAGTCGGCCTCATC20460.1822733387379843No Hit
GACCATCACCAGGGTCGAGGCCGGGGATGAGGCCGACTATTATTGTCAGGTGTGGGATGGTAATAGTCTTCATC20060.17870983260429937No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19950.17772986841753602No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19880.17710625484414116No Hit
GCACAGGCTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCA19880.17710625484414116No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19510.1738100116704826No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCGC19510.1738100116704826No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC19200.17104829441687677No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18980.16908836604335004No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18740.1669502623631391No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC18620.16588121052303362No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18180.1619613537759802No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT17500.1559033933487158No Hit
GTACATTGGCAGTAAAAGTGTCCACTGGTACCAGCAGAAGCCAGGCCAGG17310.15421072793521545No Hit
GTCTTCATCCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA17270.15385437732184695No Hit
CCCCAGGAGACACGGCCACCATTACGTGTGGGGAAAGGTACATTGGCAGT17100.15233988721503086No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16930.15082539710821477No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC16780.14948908230808292No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16720.14895455638803018No Hit
TCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC15850.14120393054726543No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15720.14004579105381784No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15700.1398676157471336No Hit
CTATTATTGTCAGGTGTGGGATGGTAATAGTCTTCATCCTGTGGTGTTCG15620.13915491452039663No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15400.1371949861468699No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA15020.13380965531986921No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14770.13158246398631612No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14760.131493376332974No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA14710.13104793806626341No Hit
GACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGACACGGCCACCATTA14710.13104793806626341No Hit
CAATAATAGTCGGCCTCATCCCCGGCCTCGACCCTGGTGATGGTCAGGGT14470.12890983438605244No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC14240.1268608183591836No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14230.12677173070584147No Hit
CCTGAGGGCCGGTCGCTATCATCATGGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACA14180.12632629243913085No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT14160.1261481171324466No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC14090.12552450355905176No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13960.12436636406560415No Hit
TCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCCTCGACCCTGGT13850.12338639987884079No Hit
GCTTGGTCCCTCCGCCGAACACCACAGGATGAAGACTATTACCATCCCACACCTGACAATAATAGTCGGCCTCAT13850.12338639987884079No Hit
CAGTAAAAGTGTCCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC13740.12240643569207743No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTC13730.12231734803873531No Hit
CTATTACCATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCCTC13650.12160464681199833No Hit
CTATCATCATGGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTA13630.12142647150531409No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG13490.12017924435852435No Hit
AGCCAGGCCAGGCCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCC13320.11866475425170826No Hit
GGTGTGGGATGGTAATAGTCTTCATCCTGTGGTGTTCGGCGGAGGGACCA13200.11759570241160278No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCCTCGACCCTGG13100.11670482587818154No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12940.11527942342470757No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG12930.11519033577136543No Hit
ATTATTGTCAGGTGTGGGATGGTAATAGTCTTCATCCTGTGGTGTTCGGC12860.11456672219797058No Hit
GGTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCT12690.11305223209115449No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC12690.11305223209115449No Hit
CTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCC12660.11278496913112812No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG12290.10948872595746956No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12160.10833058646402195No Hit
CTGCACAGGCTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGACACGGCCAC12150.10824149881067983No Hit
GACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACACTTTTACTGCCAATGTACCTTTCCCCACA12070.10752879758394285No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12040.10726153462391648No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGGATGAAGACTATTACCATCCCACACCTGACAAT12030.10717244697057435No Hit
GTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAA12030.10717244697057435No Hit
GCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATT11970.1066379210505216No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11950.10645974574383736No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC11910.10610339513046887No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC11910.10610339513046887No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11720.10441072971696852No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT11610.10343076553020517No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC11560.10298532726349455No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC11540.10280715195681031No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG11480.10227262603675756No Hit
CATCATGGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACACTTTTACTGCCAATGTAC11370.10129266184999422No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11340.10102539888996784No Hit
GCCTCATCCCCGGCCTCGACCCTGGTGATGGTCAGGGTGGCCGTGTTCCC11270.10040178531657297No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG11250.10022361000988873No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACCG150.00241428368.5726245
AGGGGGG2850.055.60613670
CTCGTAT1950.047.9151839
CGTATGC2250.044.68087441
TCGTATG2350.044.212940
GGTATCA8350.043.9612161
AATCTCG2200.042.36931636
GTATCAA24100.041.7083471
CGAGACT2550.041.69812423
GTGGTAT2900.040.2210431
GCTAATC2350.039.5637433
TCTCGTA2400.038.89436338
TATCAAC26450.038.110312
TAGACTG451.03886865E-438.0959055
CTTATAC2850.037.3154951
ACGAGAC2850.037.30718222
TATACAC3150.037.007453
ATCTCGT2600.035.87509537
CTAATCT2600.035.79316334
ATCAACG28400.035.3728563
Produced by FastQC (version 0.11.5)