FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122490
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGACACGGCCAC61300.5461073149872159No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53810.47938066263396556No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCGCTGTACTGACTCAGC50390.44891268519095934No Hit
CCATTACGTGTGGGGAAAGGTACATTGGCAGTAAAAGTGTCCACTGGTACCAGCAGAAGCCAGGCCAGGCCC46840.41728656825450566No Hit
CCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGACACG46030.41007046833379357No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG43120.38414596121123573No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43050.3835223476378409No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42830.38156241926431417No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC39390.3509162665146237No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGGATGAAGA39180.34904542579443915No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37970.3382658197400422No Hit
CCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCT37550.33452413829967304No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG36020.3208937273383282No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35730.3183101853914066No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34150.3042343361633511No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG33860.30165079421642954No Hit
GTAATAGTCTTCATCCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT32390.28855490917513743No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT31860.28383326354800487No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31000.27617172536058227No Hit
GACTATTACCATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCCTCGACCCTGGTGATGGTCAGGGTGG29600.263699453892685No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATGGA28710.255770652745236No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27810.24775276394444495No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA27350.24365473189070724No Hit
CCACAGGATGAAGACTATTACCATCCCACACCTGACAATAATAGTCGGCC26500.2360822813566268No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACACTTTTACTG26350.2347459665564949No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT26330.2345677912498107No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26240.23376600236973158No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG26000.23162789868952063No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGA25870.23046975919607302No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG25530.2274407789824408No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25210.22458997407549286No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24870.22156099386186068No Hit
GTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG24820.2211155555951501No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24730.22031376671507097No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCGCTGTACTGACT24450.2178193124214915No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24210.21568120874128055No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG24000.213810368021096No Hit
GGCCAGGCCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGG23990.21372128036775384No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23820.21220679026093775No Hit
ATCCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC22640.2016944471665672No Hit
CGGCCACCCTGACCATCACCAGGGTCGAGGCCGGGGATGAGGCCGACTAT22610.2014271842065408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22500.20044722001977747No Hit
GGCTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGC22170.19750732745948738No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGGATGAAGAC22130.1971509768461189No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21890.19501287316590793No Hit
TGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGAC21860.19474561020588155No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21690.19323112009906546No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21670.19305294479238122No Hit
ACCCTGGTGATGGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAA21300.18975670161872266No Hit
GTGTCAGTGGCCCCAGGAGACACGGCCACCATTACGTGTGGGGAAAGGTA21210.18895491273864357No Hit
CACCAGGGTCGAGGCCGGGGATGAGGCCGACTATTATTGTCAGGTGTGGG21000.18708407201845897No Hit
CTCACTGCACAGGCTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGACACGG20590.1834314782314319No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA20580.1833423905780898No Hit
GACCATCACCAGGGTCGAGGCCGGGGATGAGGCCGACTATTATTGTCAGGTGTGGGATGGTAATAGTCTTCATC20270.18058067332448394No Hit
GCACAGGCTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCA20110.17915527087101No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCGC19990.1780862190309045No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19710.17559176473732505No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19210.1711373820702189No Hit
GGATGAAGACTATTACCATCCCACACCTGACAATAATAGTCGGCCTCATC19000.1692665413500343No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18480.16463398337624388No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC18450.16436672041621753No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17910.15955598713574284No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT17820.15875419825566375No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17560.15643791926876854No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17510.15599248100205793No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC17500.1559033933487158No Hit
GTCTTCATCCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA17490.1558143056953737No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17100.15233988721503086No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16820.14984543292145142No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16690.1486872934280038No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16510.14708371566784562No Hit
GTACATTGGCAGTAAAAGTGTCCACTGGTACCAGCAGAAGCCAGGCCAGG16280.14503469964097676No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16240.14467834902760826No Hit
TCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC15700.1398676157471336No Hit
CTATTATTGTCAGGTGTGGGATGGTAATAGTCTTCATCCTGTGGTGTTCG15380.13701681084018566No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15360.1368386355335014No Hit
CCCCAGGAGACACGGCCACCATTACGTGTGGGGAAAGGTACATTGGCAGT15080.13434418123992195No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15010.1337205676665271No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14890.1326515158264216No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA14480.12899892203939456No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA14350.12784078254594694No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC14180.12632629243913085No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGGATGAAGACTATTACCATCCCA14180.12632629243913085No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTC14050.12516815294568326No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG14030.12498997763899901No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC13920.12401001345223567No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13810.1230300492654723No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACAGGATGAAGACTATTACCATCCCACACCTGACAAT13390.11928836782510312No Hit
GCTTGGTCCCTCCGCCGAACACCACAGGATGAAGACTATTACCATCCCACACCTGACAATAATAGTCGGCCTCAT13390.11928836782510312No Hit
GTGCTGGTCGTCCATGATGATAGCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAA13370.11911019251841888No Hit
CTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCC13330.11875384190505038No Hit
GGTGTGGGATGGTAATAGTCTTCATCCTGTGGTGTTCGGCGGAGGGACCA13200.11759570241160278No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG13160.11723935179823429No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC13140.11706117649155003No Hit
GACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGACACGGCCACCATTA13100.11670482587818154No Hit
CTATTACCATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCCTC13080.1165266505714973No Hit
CTGCACAGGCTCTGTGACCTCCGCTGTACTGACTCAGCCTCCCGCGGTGTCAGTGGCCCCAGGAGACACGGCCAC13010.11590303699810242No Hit
TCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCCTCGACCCTGGT12910.1150121604646812No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12750.11358675801120724No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC12720.11331949505118084No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT12620.11242861851775963No Hit
CCTGAGGGCCGGTCGCTATCATCATGGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGGACA12580.11207226790439113No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC12500.11135956667765415No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12500.11135956667765415No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12470.11109230371762777No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGG12470.11109230371762777No Hit
CTATCATCATGGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTA12370.11020142718420654No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT12350.1100232518775223No Hit
AGCCAGGCCAGGCCCCTGTGCTGGTCGTCCATGATGATAGCGACCGGCCC12260.10922146299744317No Hit
CAGTAAAAGTGTCCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC12240.10904328769075894No Hit
CAATAATAGTCGGCCTCATCCCCGGCCTCGACCCTGGTGATGGTCAGGGT12220.10886511238407469No Hit
ATTATTGTCAGGTGTGGGATGGTAATAGTCTTCATCCTGTGGTGTTCGGC12090.10770697289062708No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC11850.10556886921041612No Hit
GTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACT11740.10458890502365277No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC11710.10432164206362642No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG11580.10316350257017881No Hit
CAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC11570.10307441491683668No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCT11510.10253988899678393No Hit
GGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC11330.10093631123662572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.042.3129541
GTATCAA22500.041.6556321
TATCAAC25500.036.4671822
CAACGCA27550.033.8777435
ATCAACG27600.033.6925053
TCAACGC27750.033.6335834
GTGGTAT2550.033.5355571
AACGCAG28250.033.1593176
TTGCGAC450.004122998631.39194760
ACGCAGA30200.031.0217
CGCAGAG30750.030.8065838
TAATCGT450.004570034330.7384838
AGAGTAC30950.030.61160511
CAGAGTA31450.030.23368610
GCAGAGT32300.029.4367529
GTGTATA1800.028.5052221
AACGAAC500.007208347328.00149351
GTATAAG3450.027.7616081
GTATACC500.00806435827.3650131
TGGTATC3250.027.3503862
Produced by FastQC (version 0.11.5)