FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423329
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT9980.2357504446895913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT303.174431E-584.1688170
AATTGTC306.076964E-446.3478444
GTGGTAT3350.039.889231
TATACAC1750.039.1401563
GTATCAA15800.037.950441
TGGTATC3800.037.852652
AGGGGGG2550.037.133370
TCGTATG1900.036.44620540
TTATACA2000.034.2476352
CTTATAC2050.033.4281231
CGTATGC2150.032.24678441
TATCAAC18700.032.0499272
GGTATCA6900.031.7809411
TCTTATA652.4373763E-531.6132032
GTGTATA652.4373763E-531.61320313
ATACACA2200.031.1342164
AACGCAG19550.030.8316356
CAACGCA19750.030.3460085
TACACAT2650.029.7243655
ATAAGAT850.005391687629.70663870
Produced by FastQC (version 0.11.5)