FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423329
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19960.4715008893791826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15950.039.063061
TGGTATC2900.038.932742
GTGGTAT3000.037.657221
GGTATCA6550.037.1084371
TATCAAC19200.032.6098562
ATCAACG19350.032.1802523
AACGCAG19500.031.4100576
CAACGCA19950.030.869435
TCAACGC20350.030.7670384
AGAGTAC19800.029.74903311
CAGAGTA19850.029.67409710
TAGACAC605.6871993E-428.511355
ACGCAGA21750.028.3281047
GCAGAGT21850.028.211799
CGCAGAG22100.027.8893558
TAATCGT658.512073E-426.61048938
TACGGGG9750.023.88155215
AGTACGG23050.023.02875113
ATTACGA750.001987172823.00746735
GAGTACG23350.022.87954112
Produced by FastQC (version 0.11.5)