FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098492
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT68720.6255848927438707No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51800.47155555070041477No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48820.44442745145162643No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45750.4164800471919686No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44260.4029159975675744No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42350.3855285245591229No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41470.3775175422306216No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTC39860.36286108592506816No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38970.3547590697064703No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGC38910.35421286636589067No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37500.3413770878622694No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC37440.34083088452168975No Hit
GAATTCACTCTCGCCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA35570.3238075470736246No Hit
CCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCG33220.3024145829009223No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32610.29686151560502944No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31520.28693882158449946No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31490.2866657199142097No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31190.2839347032113115No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30780.28020231371735066No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGCCGATTAATAGTTGGTTGGCCTGGTATCAGCAGAAG29430.26791273855430897No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28420.25871831565455183No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28230.2569886717427164No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27930.25425765503981823No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26370.24005636818474782No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT26020.23687018203136664No Hit
GGTATCAGCAGAAGCCAGGGAAAGTCCCTAAGCTCCTGATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCAT25680.23377502976808207No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT24740.2252178440990012No Hit
GAATTAACATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGCGAGAGTGAATT24710.22494474242871135No Hit
ATGTTAATTCTTCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAA24700.22485370853861475No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24630.2242164713079385No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24490.22294199684658603No Hit
GATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATTCA24020.21866340401204562No Hit
CCTTATAGATCAGGAGCTTAGGGACTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAACTATTAATCGGCTGA23450.2134744722765391No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC23210.21128965891422058No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23090.21019725223306135No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC22650.20619176106881068No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21700.1975435415096332No Hit
GCACTGGATCTGGGACAGAATTCACTCTCGCCATCAGCAGCCTGCAGCCT21640.19699733816905357No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21280.1937201181255758No Hit
TCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATTCACT21090.1919904742137403No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20920.19044289808209802No Hit
CCTTGGTCCCTTGGCCGAACGTCGGAGAAGAATTAACATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGG20570.18725671192871682No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC20450.18616430524755756No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20030.18234088186350014No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA19550.17797125513886308No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19210.17487610287557853No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGACTCCTTCCACC19120.1740567978647091No Hit
CATCAAGGTTCAGCGGCACTGGATCTGGGACAGAATTCACTCTCGCCATCAGCAGCCTGCAGCCTGATGATTTTG19090.17378369619441927No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18780.1709616456014245No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18390.1674113238876569No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18200.1656816799758214No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18090.16468030718475873No Hit
GTCCCTAAGCTCCTGATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCACTGGAT18010.1639520360639859No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17770.16176722270166738No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17470.15903620599876922No Hit
ACCTTGATGGGACCCCACTTTCTAAATTAGACGCCTTATAGATCAGGAGCTTAGGGACTTTCCCTGGCTTCTG17470.15903620599876922No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC17300.15748862986712692No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17210.15666932485625748No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16960.15439347760384237No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC16790.15284590147220006No Hit
GTATGTTAATTCTTCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16790.15284590147220006No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16690.15193556257123403No Hit
CTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGG16560.15075212199997814No Hit
GTATCAGCAGAAGCCAGGGAAAGTCCCTAAGCTCCTGATCTATAAGGCGT16310.148476274747563No Hit
GAGTGAATTCTGTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCA16140.14692869861592076No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16090.1464735291654377No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15910.14483491914369884No Hit
GGATCTGGGACAGAATTCACTCTCGCCATCAGCAGCCTGCAGCCTGATGA15900.1447438852536022No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15890.1446528513635056No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC15830.14410664802292597No Hit
TTATAGATCAGGAGCTTAGGGACTTTCCCTGGCTTCTGCTGATACCAGGC15740.14328734301205653No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15660.1425590718912837No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15570.14173976688041423No Hit
ACTTATTACTGCCAACAGTATGTTAATTCTTCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAA15510.14119356353983462No Hit
GTCCCAGATCCAGTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAATT15250.13882668239732288No Hit
CCCAGACTCCTTCCACCCTGTCTGCATCTGTCGGAGACAGAGTCACCATC15090.1373701401557772No Hit
ATCCAGTGCCGCTGAACCTTGATGGGACCCCACTTTCTAAATTAGACGCC15030.13682393681519756No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15010.13664186903500436No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14930.1359135979142315No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14820.13491222512316886No Hit
GCTGATACCAGGCCAACCAACTATTAATCGGCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATG14770.13445705567268582No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTCCCAGATCCAGTGCCG14660.13345568288162318No Hit
GCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGTTAATTCTTCTCCGACGTTCGGCCAAGGGA14530.1322722423103673No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14460.13163500507969106No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14090.1282667511461167No Hit
GATTAATAGTTGGTTGGCCTGGTATCAGCAGAAGCCAGGGAAAGTCCCTA14070.12808468336592346No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC13760.1252626327729287No Hit
GGCTGCAGGCTGCTGATGGCGAGAGTGAATTCTGTCCCAGATCCAGTGCC13750.12517159888283208No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC13700.12471642943234908No Hit
CGCCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATGTTAATTCTTCTCCGACGTT13660.12435229387196264No Hit
ATCTGGGACAGAATTCACTCTCGCCATCAGCAGCCTGCAGCCTGATGATT13630.12407919220167284No Hit
CAGTATGTTAATTCTTCTCCGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13560.1234419549709966No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13430.12225851439974073No Hit
CTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGG13120.11943646380674598No Hit
GCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGACTCCTTCCACCCTGTCTGCATCTGTCGGAGA12840.11688751488404103No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC12800.1165233793236546No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12770.11625027765336479No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12720.11579510820288177No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12620.11488476930191571No Hit
GATCAGGAGCTTAGGGACTTTCCCTGGCTTCTGCTGATACCAGGCCAACC12550.11424753207123949No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAACGTCGGAGA12400.1128820237197904No Hit
GCTTAGGGACTTTCCCTGGCTTCTGCTGATACCAGGCCAACCAACTATTA12390.1127909898296938No Hit
GCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTC12300.11197168481882436No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12200.11106134591785831No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG12150.11060617646737526No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12060.10978687145650583No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC12020.10942273589611941No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT12000.1092406681159262No Hit
AACATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC12000.1092406681159262No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11990.10914963422582959No Hit
CTATTAATCGGCTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCGACAGATGCAGACAGGGTGGAAGGAGT11970.10896756644563639No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11800.1074199903139941No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG11690.10641861752293143No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAGCCGATTAATAGTTGGTTGGCC11620.10578138029225519No Hit
CTCCTGATCTATAAGGCGTCTAATTTAGAAAGTGGGGTCCCATCAAGGTT11580.10541724473186878No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11510.10478000750119255No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGA11410.10386966860022649No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGAC11360.10341449914974346No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11220.102140024688391No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG11220.102140024688391No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11210.1020489907982944No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT11190.10186692301810119No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGC11090.10095658411713514No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAG200.00683362452.72832556
CGGATAT200.00712992152.16541745
TACTAAT200.00740882351.6616434
CGTATGC1800.050.05293341
TCGTATG1950.046.1538240
CTTATAC2100.044.0776561
GTGGTAT1950.043.9520761
GTATCAA19400.043.4717751
ATCTCCG2050.043.44476710
ACGAGAC2100.042.42968422
CACGAGA2100.042.4238921
TATACAC2200.042.039653
CTCGTAT2150.041.82773639
TATCAAC21400.039.37672
TTATACA2350.039.356272
ATCTCGT2250.038.3503837
TACACAT2650.037.4860885
TAAGACA2500.037.131831
TCAACGC23000.036.339594
ATCAACG23150.036.252093
Produced by FastQC (version 0.11.5)