FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555524
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20480.36866093994138865No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15550.2799158992230759No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13860.2494941712689281No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13850.2493141610443473No Hit
GAGTTACAGTACACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT13440.24193374183653632No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13380.24085368048905176No Hit
GTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAGA13290.23923358846782497No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA13030.2345533226287253No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12960.23329325105666002No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12820.23077310791252945No Hit
GTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACACCTCCCACTTTCGGCGGAGGGA12680.22825296476839887No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA12640.22753292387007584No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12260.2206925353360071No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11530.20755178894161186No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11450.20611170714496582No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11370.2046716253483198No Hit
ACAGTACACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA10350.18631058244108267No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10270.1848705006444366No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG10250.1845104801952751No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9940.17893016323327166No Hit
GTCTCTGGTGCCTCCATCAGTGGTTACAACTGGAGTTGGCTCCGGCAACC9880.1778501018857871No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9510.17118972357629914No Hit
GTATTACTGTGCGCGACGCGTCTATAGTTCTGGTAGCTCTTACTGGTTCGACTCCTGGGGCCAGGGAACCCTGGT9470.1704696826779761No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9380.16884959065674932No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9360.16848957020758779No Hit
GTGTATTACTGTGCGCGACGCGTCTATAGTTCTGGTAGCTCTTACTGGTT9010.16218921234726133No Hit
GTGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAAT8880.15984907942771148No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT8860.15948905897854998No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTC8850.15930904875396923No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC8800.15840899763106545No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8750.15750894650816166No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC8650.1557088442623541No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG8600.15480879313945034No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT8550.15390874201654653No Hit
GCATAGATCAGGAGCTTGGGGGCTTTCCCTGGTTTCTGCTGATACCAATT8540.15372873179196578No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8080.145448261461251No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT8080.145448261461251No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT8070.14526825123667025No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGCAGGTATTTAAATTGGTATCAGCAGAAA7990.1438281694400242No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7990.1438281694400242No Hit
CACCTGCACTGTCTCTGGTGCCTCCATCAGTGGTTACAACTGGAGTTGGCTCCGGCAACCCCCAGGGAAGGGA7980.14364815921544344No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7800.14040797517298984No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7780.1400479547238283No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT7720.13896789337634377No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7600.1368077706813747No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7590.13662776045679395No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7510.1351876786601479No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7500.13500766843556716No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7490.13482765821098638No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7490.13482765821098638No Hit
GATCAGGAGCTTGGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT7310.13158747416853278No Hit
GTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA7250.13050741282104825No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA7210.12978737192272521No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAAT7110.12798726967691765No Hit
GTTACAGTACACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA7110.12798726967691765No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7010.12618716743111008No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6880.12384703451156025No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6880.12384703451156025No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG6830.12294698338865648No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6760.12168691181659119No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6670.12006681979536438No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA6620.1191667686724606No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA6610.11898675844787983No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT6430.11574657440542623No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6420.11556656418084546No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6310.11358645171045716No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC6280.11304642103671489No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6210.11178634946464958No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC6210.11178634946464958No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGGGAGGTGTA6170.11106630856632657No Hit
GGTTGTAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCC6130.11034626766800355No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC6090.10962622676968052No Hit
ACTTACTACTGTCAACAGAGTTACAGTACACCTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA6080.10944621654509976No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG6020.10836615519761522No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT5980.10764611429929222No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5970.10746610407471144No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA5940.10692607340096918No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT5830.10494596093058085No Hit
GATTTCACTCTCACCATCAGCAGTCTACAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACA5810.10458594048141934No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA5760.10368588935851557No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC5740.10332586890935405No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5720.10296584846019254No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5690.10242581778645027No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT5690.10242581778645027No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5680.1022458075618695No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT5620.10116574621438497No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5590.1006257155406427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGA350.00517791356.5485370
GTGGTAT1300.044.9308171
GTATCAA7650.044.0152741
TACCGAT5400.040.3170170
GTCTTAG451.02535145E-438.1765141
TATCAAC9000.037.0178832
ATCAACG9200.035.8398133
CAGATAG750.002354514335.18575770
TCAACGC9400.035.0772634
CAACGCA9450.034.891675
AACGCAG9650.034.8803756
GTATAAG701.0040967E-634.358861
TATAAGA701.0069289E-634.346492
GTACGGA400.00265255934.346493
ATAAGAG959.169526E-932.5387763
AGAGTAC10050.032.15691411
CGCAGAG10500.032.0711568
GCAGAGT10400.031.7330159
GTGCTAC450.004714244530.541211
GTGTATA450.004714244530.541211
Produced by FastQC (version 0.11.5)