FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206615
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCA72980.6048325273595969No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56040.4644397757362539No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATGGCCAGGGAGG55370.4588870517936542No Hit
GATTATTACTGTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGG46080.3818948048880546No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30980.2567513249876721No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTC30140.24978970094023364No Hit
CCCCAGGGACGGCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCC30010.2487123067424158No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29820.24713765368406657No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27080.22442949905313625No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26810.2221918341807453No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25920.2148158277495307No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24090.19964943250332542No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24000.19890354421252843No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23460.19442821446774655No Hit
GACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGG23310.19318506731641824No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG23270.19285356140939736No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT22280.18464879021063058No Hit
CCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG21840.18100222523340087No Hit
ATCCAATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGC21130.17511799538378026No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20900.17321183641841018No Hit
ACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGG20880.17304608346489975No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA19910.16500706521964337No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19610.16252077091698677No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG19500.16160912967267935No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGT19440.16111187081214803No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATC19300.15995160013757495No Hit
CCCCTGAGGGCCGCTGTTCACTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCAGTAGA19110.15837694707922575No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18860.15630503516034525No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17970.14892902872913066No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC17800.14752012862429192No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17410.14428794603083833No Hit
GTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA17160.14221603411195782No Hit
GGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG16930.14030987514658777No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC16750.1388180985649938No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16650.1379893337974416No Hit
CTCCAGACCCTTCCCCGGAGCCTGACGGACCCAGTGAAGGGCAAAATCAT16600.1375749514136655No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16360.1355859159715402No Hit
GTCTGAGACCTGAGGACACCGCCTTGTATTATTGTGTCAAGTCAGTGGGA16190.13417701586670147No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15940.13210510394782096No Hit
GCACTGAACACCGAACTCACCATGGCGCTTGGACTGAACTGGGTTTTCCTTGTTGCCATTTTAAAAGGTGTCCA15940.13210510394782096No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15840.13127633918026876No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15830.13119346270351354No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15560.1289557978311226No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGG15410.1277126506797943No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15250.12638662705171078No Hit
ATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATG15170.125723615237669No Hit
GTTCACTCCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGG15120.1253092328538929No Hit
ACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGGC15090.12506060342362724No Hit
GTATTATTGTGTCAAGTCAGTGGGAACTATTACGTCGGACACCCGAGACT15020.12448046808634071No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15010.1243975916095855No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14960.1239832092258094No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14910.12356882684203328No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14890.12340307388852285No Hit
GATCAGCACTGAACACCGAACTCACCATGGCGCTTGGACTGAACTGGGTT14810.12274006207448108No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC14780.12249143264421543No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAA14580.12083390310911103No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14470.1199222618648036No Hit
GCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT14420.1195078794810275No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14400.11934212652751706No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14360.11901062062049618No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14320.1186791147134753No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14220.1178503499459231No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14190.11760172051565744No Hit
CAATAATACAAGGCGGTGTCCTCAGGTCTCAGACTGCTCATTTCCAAGTA14110.11693870870161567No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13910.11528117916651127No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGTCCGAAGATG13860.11486679678273518No Hit
GTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCAGTAGACATAATTAGCT13820.11453529087571429No Hit
GCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT13680.11337502020114121No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13480.1117174906660368No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13360.11072297294497417No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13290.11014283760768763No Hit
CAACTAGGCAGGCTGTCATCCAATGTTGCACAGTAATAATCAGCCTCATC13270.10997708465417717No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13270.10997708465417717No Hit
TCCCTGGGGATTGCTGGTACCAGTAGACATAATTAGCTCCGATGTTGGAGTCGCTTCCAGAACAAGAGATGGTGA13170.10914831988662498No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTC13050.10815380216556233No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12990.10765654330503102No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCT12840.10641339615370271No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12770.10583326081641617No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12630.10467299014184309No Hit
CTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTATGTCTACTGGTACCAGCAATCCCCAGGGACGGCCCC12610.10450723718833264No Hit
GTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACC12510.10367847242078045No Hit
CTCCTGTGTGGCCTCTGGATTCCTTTTTGATGATTTTGCCCTTCACTGGGTCCGTCAGGCTCCGGGGAAGGGT12470.10334696651375956No Hit
CCCATAGATGAGGAGTTTGGGGGCCGTCCCTGGGGATTGCTGGTACCAGT12370.10251820174620736No Hit
GGCCATCAGCGGTCTCCGGTCCGAAGATGAGGCTGATTATTACTGTGCAA12230.10135793107163428No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA12210.10119217811812384No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12200.10110930164136862No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG12140.1006120427808373No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12100.10028053687381643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAACG200.00737344951.7245517
GTGGTAT2700.047.281951
CTTATAC856.548362E-1140.591821
GTATCAA15500.039.4002531
AATGTTG29950.037.0627970
GGTATCA6700.036.5629351
TGGTATC3600.036.3987582
TCGTATG806.862501E-834.63084440
CGTTATT400.002570028234.57185436
TATACAC905.2150426E-934.4830323
TATCAAC17950.034.386982
CAACGCA19300.031.8030035
ATCAACG19450.031.7350273
GTAGTTT1003.1531783E-431.53495870
TCAACGC19550.031.3963174
CGTTTAA450.004149017431.35184366
AACGCAG19900.031.01746
GCCAGAG25500.030.91662870
CGTATGC953.0838055E-729.17981541
CGCAGAG21600.028.8955028
Produced by FastQC (version 0.11.5)