FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206615
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53920.4468699626641472No Hit
GTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCC53100.44007409157021915No Hit
GCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCA50360.41736593693928886No Hit
GATTATTACTGTGCAACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGG44360.3676400508861567No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCT42230.34998736133729486No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGACCGCTGATGGCCAGGGAGG33230.2753985322575967No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28890.23943014134583113No Hit
GTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCAC28840.239015758962055No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28260.23420892331025225No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCAC27620.22890482879791815No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27440.22741305221632419No Hit
CCCCAGGGACGGCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCC25900.21465007479602027No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25900.21465007479602027No Hit
GTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA25670.21274391583065022No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25430.2107548803885249No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT24570.203627503387576No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG22840.1892898729089229No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC21950.1819138664777083No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGATCAACTTGGTCCCTC21630.17926181922154125No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21250.17611251310484288No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT21000.17404060118596237No Hit
CCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCACCATCTCTTGTTCTGG20740.17188581279032666No Hit
ACATTGGATGACAGCCTGCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGG19960.16542144760341948No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA19100.15829407060247053No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGG18910.15671941754412136No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGT18130.15025505235721417No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17490.1449509578448801No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC17260.14304479887951002No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT17240.1428790459259996No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG17100.1417187752514265No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16670.13815508675095203No Hit
GGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG16510.13682906312286852No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGGCAATCTCCAGCATGGCCGGCTTCCCTCTCCT16480.13658043369260284No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16430.13616605130882675No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16340.13542016301802975No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC16290.13500578063425367No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16180.13409413938994624No Hit
GTATTATTGTGTCAAGTCAGTGGGAACTATTACGTCGGACACCCGAGACT15720.1302818214592061No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTT15700.13011606850569568No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15680.12995031555218522No Hit
CTCCAGACCCTTCCCCGGAGCCTGACGGACCCAGTGAAGGGCAAAATCAT15600.12928730373814348No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15390.12754689772628386No Hit
GACCCAGGACGATCAACTTGGTCCCTCCGCCGAACACCCAACTAGGCAGG15270.1265523800052212No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14840.12298869150474674No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14810.12274006207448108No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14780.12249143264421543No Hit
GTCTGAGACCTGAGGACACCGCCTTGTATTATTGTGTCAAGTCAGTGGGA14720.12199417378368412No Hit
GCCCCCAAACTCCTCATCTATGGGAGTGAACAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT14700.12182842083017366No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14340.11884486766698575No Hit
GCACTGAACACCGAACTCACCATGGCGCTTGGACTGAACTGGGTTTTCCTTGTTGCCATTTTAAAAGGTGTCCA14320.1186791147134753No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTTCTGACGCAGCCACCCTCAGCCTCTGGGACCCCCGGACAGAGGGTCAC14040.11635857336432913No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14000.11602706745730826No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13990.11594419098055304No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13970.11577843802704259No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13970.11577843802704259No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13950.11561268507353216No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13910.11528117916651127No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT13910.11528117916651127No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13880.11503254973624562No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13870.11494967325949039No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13820.11453529087571429No Hit
GATCAGCACTGAACACCGAACTCACCATGGCGCTTGGACTGAACTGGGTT13770.11412090849193819No Hit
CAATAATACAAGGCGGTGTCCTCAGGTCTCAGACTGCTCATTTCCAAGTA13660.11320926724763077No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGTCTCCGGTCCGAAGATG13630.11296063781736511No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC13610.11279488486385467No Hit
GCCTAGTTGGGTGTTCGGCGGAGGGACCAAGTTGATCGTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACT13380.1108887258984846No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT13090.10848530807258322No Hit
CTCTTGTTCTGGAAGCGACTCCAACATCGGAGCTAATTATGTCTACTGGTACCAGCAATCCCCAGGGACGGCCCC12940.10724216092125492No Hit
CCCCCGGACAGAGGGTCACCATCTCTTGTTCTGGAAGCGACTCCAACATC12830.1063305196769475No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12780.1059161372931714No Hit
ATCCAATGTTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGACCGC12720.10541887843264007No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12710.10533600195588486No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12590.10434148423482222No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12560.10409285480455654No Hit
CTCCTGTGTGGCCTCTGGATTCCTTTTTGATGATTTTGCCCTTCACTGGGTCCGTCAGGCTCCGGGGAAGGGT12270.10168943697865516No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12220.10127505459487907No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12210.10119217811812384No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12090.1001976603970612No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)