FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709510
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGTCCGCTGATGGCCAGGGAGG29730.4190215782723288No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25830.3640540654818114No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC24340.343053656749024No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAGGCACTCTGG23100.3255768065284492No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAGGCACTCTGGCTGTCATCCCATC22820.321630420994771No Hit
GATTATTACTGTGCAGGATGGGATGACAGCCAGAGTGCCTGGGTGTTCGG20720.2920325294921847No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT19990.2817437386365238No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19650.27695169905991457No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18940.26694479288523065No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC18820.2652534847993686No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18540.2613070992656904No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16660.23480993925385124No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15270.21521895392594892No Hit
ATCCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCGCTGATG14490.20422545136784542No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14290.2014066045580753No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14290.2014066045580753No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14100.19872870008879367No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14050.19802398838635113No Hit
ATCCCATCCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCGC13690.19295006412876492No Hit
CCCCTGAGGGCCGCTGATTATTCCTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGTAAA13680.19280912178827642No Hit
GGATGGGATGACAGCCAGAGTGCCTGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGG13680.19280912178827642No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGACTCCGGT12210.172090597736466No Hit
GTGCAGGATGGGATGACAGCCAGAGTGCCTGGGTGTTCGGCGGAGGGACC11910.16786232752181082No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAGGCACTCTGGC11830.16673478879790277No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC11700.1649025383715522No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11510.1622246339022706No Hit
CCCAGGCACTCTGGCTGTCATCCCATCCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCGCTGATGG11460.16151992219982805No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11350.1599695564544545No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11210.1579963636876154No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11040.1556003438993108No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10610.14953982325830503No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10240.1443249566602303No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9990.14080139814801765No Hit
CTCTTGTTCTGGGAGCAACTCCAACATCGGAAGTAATTTTGTTTACTGGTACCAGCAATTCCCAGGAACGGCCCC9820.13840537835971303No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9710.1368550126143395No Hit
CTCTGGCTGTCATCCCATCCTGCACAGTAATAATCAGCCTCATCCTCGGA9540.13445899282603488No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9390.13234485771870727No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA9140.12882129920649463No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC9070.12783470282307507No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8970.12642527941819No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8930.125861510056236No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8750.12332454792744288No Hit
GATTATTCCTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGG8710.12276077856548885No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8610.1213513551606038No Hit
TTCCTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCA8560.12064664345816127No Hit
GCCTGGTCCCTCCGCCGAACACCCAGGCACTCTGGCTGTCATCCCATCCTGCACAGTAATAATCAGCCTCATCCT8560.12064664345816127No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8300.11698214260546011No Hit
GTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGTAAACAAAATTACTT8220.11585460388155205No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT8160.115008949838621No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG8100.11416329579568998No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC8070.11374046877422447No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7980.11247198770982791No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA7920.11162633366689687No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGGAGCAA7810.11007596792152331No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7760.10937125621908077No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG7690.10838465983566124No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGACTCCGGTCCGAGGATG7620.10739806345224168No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG7600.10711617877126468No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7600.10711617877126468No Hit
GTGCCTGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCC7390.10415638962100605No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG7350.10359262025905203No Hit
GTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC7340.10345167791856351No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC7130.10049188876830488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.043.1311261
TATCAAC11000.040.4306222
TGTATAT350.001352140739.4008263
GTGGTAT1450.038.0743751
CAACGCA11800.037.689565
AACGCAG12100.037.040036
TCAACGC12050.036.907624
ATCAACG12250.036.3050463
GGTATCA4050.035.7828641
ATACAGT501.8684157E-434.475726
CGCAGAG13650.032.5814558
TGGTATC1700.032.447742
ACGCAGA14100.032.0306367
AGAGTAC13700.031.95924611
CAGAGTA13900.031.49940110
GCAGAGT14650.030.592799
TCTTATA500.007759565527.5805762
TACACTA500.007759565527.5805765
GTGCTAC658.6466345E-426.5422331
GTATAAG1850.026.1118181
Produced by FastQC (version 0.11.5)