FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709510
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25650.3615171033530183No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGTCCGCTGATGGCCAGGGAGG25250.35587940973347804No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC23840.33600653972459865No Hit
GATTATTACTGTGCAGGATGGGATGACAGCCAGAGTGCCTGGGTGTTCGG20380.28724048991557555No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC19790.2789248918267537No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18810.26511254245888005No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT18720.2638440613944835No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAGGCACTCTGGCTGTCATCCCATC18190.2563741173485927No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17800.250877366069541No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAGGCACTCTGG17760.2503135967075869No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17550.24735380755732828No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16480.23227297712505815No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14690.20704429817761552No Hit
ATCCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCGCTGATG13780.19421854519316148No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13560.19111781370241435No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13410.18900367859508674No Hit
ATCCCATCCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCGC13190.18590294710433963No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG13120.18491635072092005No Hit
CCCCTGAGGGCCGCTGATTATTCCTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGTAAA13100.18463446603994307No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12970.18280221561359247No Hit
GGATGGGATGACAGCCAGAGTGCCTGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGG12930.18223844625163846No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11550.1627884032642246No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11480.16180180688080506No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGACTCCGGT11450.16137897985933955No Hit
GTGCAGGATGGGATGACAGCCAGAGTGCCTGGGTGTTCGGCGGAGGGACC11450.16137897985933955No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA11410.1608152104973855No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA10900.1536271511324717No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10780.15193584304660962No Hit
CTCTTGTTCTGGGAGCAACTCCAACATCGGAAGTAATTTTGTTTACTGGTACCAGCAATTCCCAGGAACGGCCCC10680.15052641964172456No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10280.14488872602218433No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCAGGCACTCTGGC10100.14235176389339121No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC9770.13770066665727052No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9760.13755972431678198No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9680.13643218559287396No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9420.13276768474017278No Hit
CTCTGGCTGTCATCCCATCCTGCACAGTAATAATCAGCCTCATCCTCGGA9140.12882129920649463No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9110.12839847218502912No Hit
CCCAGGCACTCTGGCTGTCATCCCATCCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGTCCGCTGATGG9040.12741187580160956No Hit
GATTATTCCTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGG8880.12515679835379345No Hit
GTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCAGTAAACAAAATTACTT8860.12487491367281646No Hit
TTCCTATACATGAGGAGTTTGGGGGCCGTTCCTGGGAATTGCTGGTACCA8710.12276077856548885No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG8540.12036475877718425No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA8500.11980098941523022No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8460.1192372200532762No Hit
GCCTGGTCCCTCCGCCGAACACCCAGGCACTCTGGCTGTCATCCCATCCTGCACAGTAATAATCAGCCTCATCCT8360.11782779664839114No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT8340.11754591196741412No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8200.11557271920057506No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA8090.11402235345520148No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC7990.11261293005031643No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGCGGACTCCGGTCCGAGGATG7960.1121901030288509No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7880.11106256430494285No Hit
GTATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC7850.11063973728347733No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG7760.10937125621908077No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT7690.10838465983566124No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG7650.10782089047370721No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC7410.10443827430198305No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT7320.10316979323758652No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG7300.1028879085566095No Hit
GTGCCTGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCC7270.10246508153514398No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7260.10232413919465547No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGGAGCAA7220.10176036983270144No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG7140.1006328311087934No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTA352.3133047E-549.135762
GTGGTAT1500.045.8826751
GTATCAA10550.039.793981
GTATTAA350.001364491339.3280071
GGTATCA4750.039.1210171
TGGTATC1850.037.183822
CTAGTAG501.894319E-434.395033
TATCAAC12100.034.1107752
AACGCAG13350.030.9190626
TCAACGC13500.030.8281384
ATAGTAC450.00469091430.573363
CAACGCA13550.030.4605455
ATCAACG13750.030.267633
CAGAGTA14900.027.95320510
AGAGTAC14950.027.62946911
GCGGATT500.00781914827.53737311
TACGCCG950.00798649627.41479170
CGCAGAG15400.027.0399138
ACGCAGA15450.026.9448057
GCAGAGT15650.026.6135929
Produced by FastQC (version 0.11.5)