FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598634
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11650.040.2243961
GTGGTAT2300.038.951311
TATCAAC13650.033.8145452
GGTATCA5400.033.1807441
TGGTATC2700.033.169662
CAACGCA14300.032.7592775
CTCGTAT1001.389526E-831.18057839
TCAACGC15050.031.1267554
ATCAACG15050.030.897883
AACGCAG15250.030.7185336
GTATTAG953.234636E-729.0163611
ACGCAGA16000.028.8480347
TCGTATG1103.4966433E-828.35789540
AGAGTAC16050.028.32893811
TATTAGA1353.110472E-1028.0666352
CAGAGTA16350.028.0198210
CGCAGAG16650.027.7218348
TTAGAAT500.007791980627.5563324
TACTACG500.007791980627.5563322
GCAGAGT18200.025.9286929
Produced by FastQC (version 0.11.5)