FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598634
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7580.12662160852875046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11400.044.0218621
TATCAAC13550.036.5142332
GGTATCA4500.035.137081
ATCAACG14800.033.894573
TCAACGC14900.033.2058944
CAACGCA15150.032.6579445
AACGCAG15400.032.3508956
GTGGTAT1750.029.4627721
CAGAGTA16400.029.14071810
AGAGTAC16350.029.01954711
ACGCAGA17100.028.7424667
CGCAGAG17850.027.7343048
ATTAGAC500.00788820327.4871043
GCAGAGT18300.026.8666959
TATACTG907.1220875E-626.7235745
ATAAGAG1901.8189894E-1225.3170683
TATACAG700.001365320524.5420575
TTAACGG902.0970391E-423.0445135
TTTAACG902.1114071E-423.02129634
TAGATTG902.1845046E-422.905925
Produced by FastQC (version 0.11.5)