FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901258
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5000.039.1627961
GTATCAA28300.038.1164131
TCGTATG1850.037.4338340
GGTATCA11950.035.9344441
CTCGTAT1600.034.60112439
TGGTATC5950.032.8882
TATCAAC32800.032.6558042
ATCAACG33500.031.8709623
TCAACGC33550.031.8234654
CAACGCA33550.031.7211385
AACGCAG34300.031.0275276
CGTATGC2300.030.14017941
AGACGTA2300.029.8691425
ACGTAGA2200.029.67537327
ACGCAGA36100.029.5755447
CAGAGTA37100.028.4082210
TCTCGTA1950.028.3652938
AGAGTAC37400.028.27214211
CGCAGAG38800.027.6059368
TTATACA2750.027.4643672
Produced by FastQC (version 0.11.5)