FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901258
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20980.2327857283929796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12900.036.9504241
GTGGTAT5700.034.8937341
GTATCAA27050.034.7358251
TGGTATC6100.030.910552
TATCAAC30900.030.3994642
ATCAACG33000.027.841633
TCAACGC33150.027.7156524
CAACGCA33650.027.100075
AACGCAG34100.026.5413746
TGCGACG1208.9240423E-426.39703270
ACGCAGA35150.025.7513877
AGAGTAC35050.025.5441511
CAGAGTA35300.025.26606610
CGCAGAG36900.024.448058
GCAGAGT39250.023.0729549
AGTACGG35250.022.18782413
TCTAATG1103.4291592E-521.8162063
GAGTACG36150.021.6354312
AATGTAT900.00504596719.59487760
TACGGGG17750.019.32701315
Produced by FastQC (version 0.11.5)