FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879380
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATTGGACGACAGTCTGACTAGTTGGGTGTTCGG35040.3984625531624554No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC27560.3134026245764061No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC27010.307148218062726No Hit
GCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCCA26920.30612476972412384No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT20310.2309581750778958No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20140.22902499488275832No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC19640.22333917077941276No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19450.22117855762014146No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19260.21901794446087014No Hit
GACCTAGGACGATCAGCTTGGTCCCTCCGCCGAACACCCAACTAGTCAGA18560.2110577907161864No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG17610.20025472491982987No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGATCAGCTTGGTCCCTC16650.18933794264140644No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC16080.18285610316359252No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG16060.18262867019945872No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG15730.17887602629125066No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT15620.17762514498851464No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA15440.17557824831131025No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15070.17137073847483456No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13250.15067433873865677No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG13110.14908230798972003No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13000.147831426686984No Hit
CCCCTGAGGGCCGCTGATTAGTCCCATAGATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGGTACCAGTATA12210.13884782460369804No Hit
ACATTGGACGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACCAAGCTGATCGTCCTAGGTCAGCCCAAGG11870.1349814642134231No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGTCTCCA11860.13486774773135618No Hit
GGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11810.13429916532102162No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT11700.1330482840182856No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11410.1297505060383452No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11350.12906820714594375No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11300.1284996247356092No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10990.12497441379153494No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10980.12486069730946803No Hit
ATGTATACTGGTACCAGCAATTCCCAGGAATGGCCCCCAAACTCCTCATC10920.12417839841706657No Hit
GATTAGTCCCATAGATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGG10730.12201778525779527No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10490.11928858968818941No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT10480.11917487320612248No Hit
CCACCACACCCTCCAAGCAAAGCAACAACAAGTACGCGGCCAGCAGCTAC10450.11883372375992177No Hit
GTGCAACATTGGACGACAGTCTGACTAGTTGGGTGTTCGGCGGAGGGACC10350.11769655893925267No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC10310.11724169301098501No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAA10050.11428506447724532No Hit
CTATGGGACTAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC9970.11337533262071005No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9920.1128067502103755No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9830.11178330187177331No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG9630.10950897223043507No Hit
GTCCCATAGATGAGGAGTTTGGGGGCCATTCCTGGGAATTGCTGGTACCA9570.10882667333803361No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9550.1085992403738998No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9510.10814437444563216No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9410.10700720962496305No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATG9340.10621119425049466No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC9330.10609747776842776No Hit
GCTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9250.10518774591189246No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC9130.10382314812708955No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG8990.1022311173781528No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8970.10200368441401896No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC8880.10098023607541676No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG350.005683338355.2385370
GGTATCA6400.044.610561
GTATCAA15450.041.6344381
TATCAAC16900.037.837182
ATCAACG18050.035.6169663
TCAACGC18200.035.323424
CAACGCA18250.035.2266465
TACGATT501.8777896E-434.44750227
GTGGTAT2000.034.3985061
AACGCAG19100.033.6589666
TATAAGA1900.032.5695272
AGAGTAC20850.030.6986611
ACGCAGA21250.030.4239047
CAGAGTA21150.030.10051210
CGCAGAG21650.030.0308888
GTATAAG2300.029.9117431
CGTAAAC703.9053506E-529.50961522
AATAGCG604.8448434E-429.30735662
GCAGAGT22300.029.1655039
GTCGTAA605.500769E-428.67847320
Produced by FastQC (version 0.11.5)