FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences853229
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21920.25690641082288573No Hit
GAGTTACAGTAGCCTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT16130.18904655139476037No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA14290.1674814147198466No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13890.16279334152964794No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13590.15927728663699897No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13000.15236237868145597No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12430.1456818743854229No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11850.13888416825963487No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11620.13618852617527066No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11520.135016507877721No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC11500.13478210421821105No Hit
CTGTAACTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC11360.13314127860164152No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11000.12892201273046275No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCCCAGTATTAGCAACTATTTAAATTGGTATCAGCAGAAA10490.12294471941295948No Hit
ACAGTAGCCTGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA10480.12282751758320451No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10060.11790504073349592No Hit
GGCTACTGTAACTCTGCTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT9940.11649861877643633No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC9700.11368577486231715No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT9430.11052132545893306No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9360.1097009126506483No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT9230.10817728886383374No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8770.1027860046951053No Hit
ATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA8670.10161398639755563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18600.038.6591951
TATCAAC20700.034.5507772
ATCAACG21250.033.3329053
TCAACGC21150.033.327934
CAACGCA21450.032.8618055
AACGCAG21800.032.6496626
ACGCAGA23850.029.9892277
CGCAGAG24600.029.0800348
AGAGTAC24450.028.55845811
GTGGTAT3300.028.1494081
TTACACC1005.164093E-727.5077344
TTCGGGG4700.027.39484470
CAGAGTA25900.027.35804210
GTCTAAG809.740176E-525.8036251
TATAAGA2700.025.4701252
GAGTACG22950.023.53068412
AGTACGG22950.023.38080813
GTGTATA1803.5470293E-1022.9365541
GTCTTAA750.00202486422.9365541
TGTTAGA3704.7293724E-1122.61925570
Produced by FastQC (version 0.11.5)