FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005755044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163189
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA50190.4314861987174913No Hit
GTATAGATGAGGAGTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATT39510.3396696495582403No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36040.3098378681366485No Hit
TCTATACTGCGTCCAGTTTGCAAAGTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT31990.27501979471951676No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27320.2348715471002563No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25440.2187090833905754No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24820.2133789091884466No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24780.21303502698185764No Hit
GAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT24750.21277711532691593No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24500.21062785153573493No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23180.1992797387182994No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23030.19799018044359085No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTC20610.17718530694495907No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19820.1703936333648272No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTT19270.16566525302422908No Hit
CAGTATAGATGAGGAGTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATTTAAATAGTTCCTAATGTTCTGA19080.16403181254293153No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18140.1559505806880911No Hit
GAATTCACTCTCACCATCACCAGTCTGCAACCTGAAGATTTTGCATCTTACTACTGTCAACAGAGTTACAGTAC17680.15199593531231814No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17580.15113622979584573No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17390.1495027893145482No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16840.14477440897395008No Hit
TCTTACTACTGTCAACAGAGTTACAGTACCTCTACGACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA16580.14253917463112187No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAACATTAGGAA16480.14167946911464946No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG16380.14081976359817708No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16290.14004602863335192No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16090.13832661760040715No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAACATTAGGAACTATTTAAATTGGTATCAGCAGAAA15910.13677914767075686No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15450.13282450229498388No Hit
CTGTAACTCTGTTGACAGTAGTAAGATGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAATTCTGTC15430.1326525611916894No Hit
GTAGTAAGATGCAAAATCTTCAGGTTGCAGACTGGTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCA15380.1322227084334532No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14960.1286119452642692No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTCGTAGAGGTA14460.12431341768190723No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14200.12207818333907904No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13560.11657606803365575No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13530.11631815637871404No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13460.11571636251718337No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13410.11528650975894718No Hit
GTGGGGTCCCATCGAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCACT13370.1149426275523582No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12880.11073007052164352No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC12520.10763513066234293No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12480.10729124845575395No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12240.10522795521622023No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCACTGAACCTCGATGGGACCCCA12140.10436824969974785No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12140.10436824969974785No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA12140.10436824969974785No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC12080.10385242638986442No Hit
GTTTAGGGGCTTTTCCCGGTTTCTGCTGATACCAATTTAAATAGTTCCTA11950.10273480921845031No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11800.10144525094374172No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)