FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500480
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT10670.2131953324808184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC2200.040.3178331
TTATACA2200.040.285612
TATACAC2250.039.3903733
GTATCAA16200.038.7481
AGGGGGG3350.037.82591270
CTCGTAT2400.037.47131739
GGTATCA7200.037.431831
TCGTATG2500.037.40152440
TACACAT2600.035.398895
GTATGCC2800.033.4519442
TATCAAC19050.033.28262
ACGAGAC2900.032.9321222
CGTATGC2850.032.84502441
TCTCGTA2750.032.6558938
ATACACA3250.032.5145384
GTGGTAT2550.032.108321
AACGCAG20450.031.1707696
CAACGCA20600.030.7783245
ATCAACG20900.030.4996283
CTCTCTA3050.030.29718429
Produced by FastQC (version 0.11.5)