FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500480
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21530.43018702046035806No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA300.00299039564.95486570
TAACCGA200.007435387551.61071435
GTCTTAC408.9582136E-751.1412431
GTATCAA19050.035.7943951
GGTATCA9050.034.659261
GTGGTAT4100.034.0941661
TGGTATC4300.032.492152
TATCAAC22300.030.7152612
AACGCAG22100.030.6848376
CAACGCA22650.029.7892825
ATCAACG23250.029.1670933
TCAACGC23600.028.734534
CAGAGTA22400.028.46253810
AGAGTAC22650.028.30173911
CGCAGAG24050.027.9246528
ACGCAGA24200.027.8868627
GCAGAGT24150.027.3882719
GTCCTAG500.00819027827.2753281
AGTACGG24600.024.67222613
GTACGGT700.001427914824.3529721
Produced by FastQC (version 0.11.5)