FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911898
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC30830.33808605787050744No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT24340.26691581733921993No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC23500.2577042607835525No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17860.19585523819549994No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17350.190262507429559No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGA16980.18620503608956263No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGACTGTCATCCCATG16560.18159925781172895No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC16190.17754178647173258No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15260.16734327742795796No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC15080.16536937245174352No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC15080.16536937245174352No Hit
GTAATAGAGGGCCGTGTCCGCAGCGGTCACAGAGCTCAGCTTCAGGGAGA14970.16416309718850136No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14840.16273749915012425No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14210.1558288317333737No Hit
ATAATAATCAGCCTCATCCCCAGACTGGAGTCCACTGATGGCCAGGGAGG13660.14979745541716288No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13650.14968779402959542No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13650.14968779402959542No Hit
CCTCAGGGCTCCCTGACCGGTTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT13570.14881050292905568No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13410.14705592072797616No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12740.13970860776095573No Hit
GCCCTGAGGGCCGCTGATTATTCCTATAAATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGGTCA12300.13488350670798707No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11690.12819416206637146No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11320.12413669072637509No Hit
GATTATTATTGTGCAGCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTA11270.12358838378853776No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11180.12260143130043052No Hit
GCATGGGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGG11170.12249176991286306No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11140.12216278575016065No Hit
CTCTATTACTGTGCGAGAAGCATAACTGGGGATCCAGGCTACTGGGGCCC10680.11711836192205707No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC10530.11547344110854503No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG10190.11174495393125108No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10170.11152563115611615No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10000.10966138756746915No Hit
GTCATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTC9980.10944206479233425No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA9880.10834545091665954No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9810.10757782120368725No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA9810.10757782120368725No Hit
ATCCCATGCTGCACAATAATAATCAGCCTCATCCCCAGACTGGAGTCCAC9660.10593290039017521No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9610.10538459345233787No Hit
ACGTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCGGACCATTCAGAC9600.10527493206477041No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9490.10406865680152824No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9490.10406865680152824No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9230.10121746072477404No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9200.10088847656207164No Hit
GGATGACAGTCTGAATGGTCCGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCC9150.1003401696242343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2850.047.1858631
CGAACTA404.9842507E-543.1498229
GTATCAA17050.040.8525471
TGGTATC3350.039.0924382
TACTGAT7650.037.4836170
GGTATCA7300.037.3161131
TATCAAC18950.036.736362
ATCAACG19800.034.8111843
CAACGCA20450.033.7047125
TCAACGC20750.033.3835034
GTCTACT4750.032.89407367
AACGCAG21400.032.369526
AGAGTAC21750.031.55595211
TGTACCG553.277019E-431.3300635
CAGAGTA22150.031.14180210
TCTACTG5300.030.85606668
TGTCTAC5050.030.6765266
CGCAGAG22750.030.6186438
ACGCAGA22900.030.2558827
GCAGAGT23400.030.0678089
Produced by FastQC (version 0.11.5)