FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526511
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT9650.18328202069852292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACTA350.006518860353.35095270
AGGGGGG2650.044.62689670
CTTATAC1700.044.5208361
GTATCAA20050.041.1800041
TTATACA2050.040.233982
TATACAC1800.040.094283
TCGTATG1900.038.35558740
GGTATCA8000.037.4126781
CTCGTAT1800.036.59200339
TATCAAC23400.035.1008532
CGTATGC2100.034.75266341
TGTATAC400.002644860434.3665282
TCAACGC25400.032.742914
CAACGCA25500.032.4797365
ATCAACG25550.032.4161763
AACGCAG25850.032.039976
TCTCGTA2200.031.48749538
CAGAGTA26200.030.69376810
AGAGTAC26300.030.5770611
ACGCAGA27150.030.5058297
Produced by FastQC (version 0.11.5)