FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031190
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49200.4771186687225438No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40620.3939138277136124No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40110.38896808541587874No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34930.3387348597251719No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34220.3318496106440132No Hit
GACTTACAGGACGCCCTGGATGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT33140.32137627401351837No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31160.3021751568576111No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30540.2961626858289937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30210.2929624996363425No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29770.28869558471280754No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT29090.2821012616491626No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28800.2792889768131964No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACATC28100.272500703071209No Hit
CCTTAGGGGCTTTCCCTGGTAACTGCTGATACCAATTTAAATAGTTGCTA25220.24457180538988935No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25060.24302019996314939No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24790.24040186580552564No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG22580.21897031584867968No Hit
CATTTATGGTGCATCCACTTTACAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA22110.214412474907631No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22010.2134427215159185No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21710.21053346134078105No Hit
CCCTGGATGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG21540.2088848805748698No Hit
CCATAAATGAGGACCTTAGGGGCTTTCCCTGGTAACTGCTGATACCAATT21310.20665444777393108No Hit
GTAGGGGACAGAGTCACCATCACTTGCCGGGCGAGTCAGAGCATTAGCAACTATTTAAATTGGTATCAGCAGTTA21060.20423006429464985No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19770.19172024554155878No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19470.18881098536642132No Hit
ACTTACTACTGTCAACAGACTTACAGGACGCCCTGGATGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA18690.1812469089110639No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18610.18047110619769394No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17740.17203425168979528No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAAATCTGTC17710.17174332567228154No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17290.1676703614270891No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17010.1649550519302941No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16950.16437319989526664No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16850.16340344650355415No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCGAGTCAGAGCATTAGCAA16720.16214276709432793No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16630.1612699890417867No Hit
GTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGGACGCCCTGGATGTTCGGCCAAGGGA16470.1597183836150467No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC16460.15962140827587543No Hit
CATCCAGGGCGTCCTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTT16120.15632424674405299No Hit
CCCCTAAGGTCCTCATTTATGGTGCATCCACTTTACAAGGTGGGGTCCCA16040.155548444030683No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA15760.15283313453388803No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15680.15205733182051803No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15660.15186338114217554No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15570.1509906030896343No Hit
GATTTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGGACG15170.14711158952278436No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACATCCAGGGCGTC15160.1470146141836131No Hit
CTTACAGGACGCCCTGGATGTTCGGCCAAGGGACCAAGGTGGAAATCAAA15020.14565695943521562No Hit
CACCATCAGCAGTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGGACGCCCTGGATGTT14620.14177794586836567No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14610.14168097052919443No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14440.1400323897632832No Hit
TTACAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTT14370.13935356238908445No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14330.13896566103239946No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14280.13848078433654323No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13950.135280598143892No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13770.13353504203880953No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13500.1309167078811858No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13420.13014090516781582No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13250.12849232440190458No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13240.12839534906273334No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12990.12597096558345214No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12650.12267380405162967No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12590.12209195201660218No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12280.11908571650229346No Hit
GTCCCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT12250.11879479048477971No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA12230.11860083980643722No Hit
CTTTACAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGT12170.11801898777140972No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12130.11763108641472474No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT12060.11695225904052599No Hit
GTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCGAGTCAGA11600.11249139343864854No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11570.1122004674211348No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11500.11152164004693606No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC11420.11074583733356608No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11380.11035793597688108No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11320.10977608394185359No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11270.10929120724599735No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11220.1088063305501411No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATTTTGCAACTTA11200.10861237987179861No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11180.10841842919345611No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11020.10686682376671612No Hit
CATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATTTTG10960.10628497173168863No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACCTTGTAAAGT10780.10453941562660615No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10770.1044424402874349No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10740.10415151426992117No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10670.10347268689572242No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG10490.10172713079063993No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC10440.10124225409478368No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10330.10017552536389995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15800.041.122331
TATCAAC18250.035.770092
CTACGCT1300.034.24464428
GTGGTAT1650.033.1602061
CTCGTAT1154.5474735E-1133.05768639
ATCAACG20000.032.64023
TCAACGC20250.032.406024
CAACGCA20250.032.237245
AACGCAG20400.031.8326596
CGAGACT1550.030.8946223
TCGTATG1251.2187229E-1030.43993840
AGAGTAC21050.030.20023711
CGCAGAG22350.028.9023918
TGTTAGA13850.027.92185670
CTTATAC1353.4015102E-1027.8637851
GCAGAGT23250.027.7835889
ACGCAGA23650.027.6027097
GTCTAGC500.00807534327.357171
CGTATGC1456.730261E-1026.2722741
AGTACGG20100.026.0162613
Produced by FastQC (version 0.11.5)