FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031190
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46490.45083835180713544No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41490.40235068222151105No Hit
GACTTACAGGACGCCCTGGATGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT33430.32418855884948455No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32190.31216361679224974No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31300.3035328116060086No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31280.3033388609276661No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT30080.29170182022711627No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29680.28782280666026633No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29050.2817133602924776No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27400.26571242932922157No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27180.2635789718674541No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACATC25840.2505842764185068No Hit
CCTTAGGGGCTTTCCCTGGTAACTGCTGATACCAATTTAAATAGTTGCTA25710.24932359700928056No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT24590.2384623590221007No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23090.22391605814641338No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22710.2202309952579059No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21750.21092136269746606No Hit
CATTTATGGTGCATCCACTTTACAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA21740.21082438735829478No Hit
CCCTGGATGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG21720.2106304366799523No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21580.2092727819315548No Hit
GTAGGGGACAGAGTCACCATCACTTGCCGGGCGAGTCAGAGCATTAGCAACTATTTAAATTGGTATCAGCAGTTA21190.2054907437038761No Hit
CCATAAATGAGGACCTTAGGGGCTTTCCCTGGTAACTGCTGATACCAATT20160.1955022837692375No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19570.1897807387581338No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19490.18900493604476382No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19290.18706542926133884No Hit
ACTTACTACTGTCAACAGACTTACAGGACGCCCTGGATGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA18870.18299246501614638No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18260.17707696932670022No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17650.17116147363725404No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG17530.16999776956719906No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17170.16650665735703413No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAAATCTGTC16960.16447017523443788No Hit
GTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGGACGCCCTGGATGTTCGGCCAAGGGA16820.1631125204860404No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16800.1629185698076979No Hit
GATTTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGGACG16590.16088208768510168No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16230.15739097547493672No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16040.155548444030683No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCGAGTCAGAGCATTAGCAA15940.1545786906389705No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15890.15409381394311425No Hit
CCCCTAAGGTCCTCATTTATGGTGCATCCACTTTACAAGGTGGGGTCCCA15860.1538028879256005No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15420.1495359730020656No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15090.14633578680941436No Hit
CACCATCAGCAGTCTTCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGGACGCCCTGGATGTT14990.14536603341770188No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT14930.1447841813826744No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGAAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA14870.1442023293476469No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14860.14410535400847566No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14760.14313560061676317No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14500.1406142417983107No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACATCCAGGGCGTC14430.13993541442411195No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14160.13731708026648826No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14160.13731708026648826No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14060.13634732687477574No Hit
CATCCAGGGCGTCCTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTT13910.134892696787207No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13720.1330501653429533No Hit
TTACAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTT13700.13285621466461078No Hit
CTTACAGGACGCCCTGGATGTTCGGCCAAGGGACCAAGGTGGAAATCAAA13690.13275923932543954No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13380.12975300381113083No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC13160.12761954634936337No Hit
CTTTACAAGGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGT12870.12480726151339713No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT12780.1239344834608559No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12770.12383750812168465No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12570.12189800133825968No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12570.12189800133825968No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12500.12121917396406094No Hit
GTCTGCATCTGTAGGGGACAGAGTCACCATCACTTGCCGGGCGAGTCAGA12380.12005546989400596No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCA12260.11889176582395096No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACCTTGTAAAGT11970.11607948098798476No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTTCAACCTGAAGATTTTGCAACTTA11740.11384904818704604No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC11600.11249139343864854No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT11590.1123944180994773No Hit
GTCCCCTACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT11530.11181256606444981No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11440.11093978801190857No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10970.10638194707085988No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC10930.10599404571417488No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10700.10376361291323617No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10660.10337571155655118No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10630.10308478553903742No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA10390.10075737739892744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAT200.007471676551.55114433
GTATCAA16100.040.72431
TATCAAC17850.036.9069252
GTGGTAT2200.035.7012561
ATCAACG18200.035.6345253
CAACGCA18350.034.9711955
GTCTAGA400.00272969434.1490251
TCAACGC19050.034.044534
AACGCAG19000.033.595166
AGAGTAC19750.032.69676611
CGCAGAG20400.031.3199848
GTATATA902.181896E-730.3546871
TATACTT450.004869920730.341445
GCAGAGT21050.030.1905469
ACGCAGA21150.030.1902987
TGTTAGA13600.029.34672470
TATAAGA2700.027.8129882
CTGCGTA500.00810907327.3338059
GAGTACG20800.026.28250512
GCGTCAA659.1604114E-426.28250511
Produced by FastQC (version 0.11.5)