FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048956
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGGCG57970.5526447248502321No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTACCGGCATCCCACAC49710.4738997631931178No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTA47530.45311719462017475No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38960.37141691357883455No Hit
CTCTAGTAGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAG31310.2984872578068098No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30910.29467394247232487No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC30260.28847730505378677No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29720.2833293293522321No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC28450.2712220531652424No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27420.26140276617894365No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG27230.2595914413950633No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG26790.2553967945271298No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25950.24738883232471146No Hit
ATATTACTGTGCGCGAGAATCCCGGGGCCACTGGGGGCAAAGAGGTCCGT24760.2360442192046187No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATGCTGACT23640.2253669362680608No Hit
CAGTAATATAGGGCCGTGTCCGCAGCGGTCACAGAGCCCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCTA23410.22317427995073197No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATGCTGACTCAGCCAC22890.2182169700159015No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22250.2121156654807256No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22150.21116233664710435No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT21860.20839768302960276No Hit
GCCCTCAGGGATCCCTGAGCGGTTCTCTGGCTCCAACTCTGGGAACACGG21310.20315437444468595No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21140.20153371542752985No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20750.197815732976407No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20610.19648107260933728No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTAC20290.19343042034174932No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19930.18999843654071286No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19880.18952177212390223No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAACCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA19330.18427846353898544No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA19040.18151380992148383No Hit
GCACAGGCTCTGTGACCTCCTATATGCTGACTCAGCCACCCTCGGTGTCA18630.17760516170363677No Hit
CTACTAGAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTA18580.17712849728682614No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18410.17550783826967004No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG18320.17464984231941094No Hit
CCCAATGATCACTACCGGCATCCCACACCTGACAGTGATAGTCGGCCTCA17700.16873920355095925No Hit
GGCTCTGTGACCTCCTATATGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGGCGGCCAGCATCAC17500.16683254588371677No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17440.16626054858354403No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA16950.16158923729879995No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA16890.1610172399986272No Hit
GTAATATAGGGCCGTGTCCGCAGCGGTCACAGAGCCCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA16860.16073124134854083No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16440.15672726024733163No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATGCTGACTCAGC16420.1565365944806074No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG16280.15520193411353764No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15950.15205594896258756No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15820.15081662147887998No Hit
GTGTCAGTGGCCCCAGGAAAGGCGGCCAGCATCACCTGTGGGGGAAACAA15800.1506259557121557No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15790.15053062282879356No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC15660.14929129534508598No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT15310.14595464442741163No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15160.1445246511769798No Hit
GCATCACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCT15020.14318999080991004No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14990.14290399215982366No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATATGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGGCGG14830.1413786660260297No Hit
GGCCAGGCCCCTCTAGTAGTCGTCTATGATGATAGCGACCGGCCCTCAGG14790.1409973344925812No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14770.14080666872585695No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14620.1393766754754251No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14530.13851867952516597No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14440.13766068357490685No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14430.13756535069154474No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14340.13670735474128562No Hit
GTGATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG14020.13365670247369765No Hit
CTATATTACTGTGCGCGAGAATCCCGGGGCCACTGGGGGCAAAGAGGTCCGTCCAACTGGTTCGACTCCTGGGGC13950.13298937229016278No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13880.13232204210662793No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13850.13203604345654155No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT13640.13003405290593695No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13390.12765073082188386No Hit
GTTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGG13290.1266974019882626No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13280.1266020691049005No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC13250.1263160704548141No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13070.12460007855429589No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13020.12412341413748527No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13000.12393274837076101No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12980.12374208260403678No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12750.12154942628670794No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCA12550.11964276861946543No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGGCGGCCAGCATCACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTG12490.11907077131929271No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12430.11849877401911996No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12400.11821277536903359No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12380.11802210960230936No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12330.11754544518549873No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTCCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12270.11697344788532597No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC12180.11611545193506688No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11880.11325546543420316No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC11840.11287413390075465No Hit
CCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAG11830.11277880101739253No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11810.1125881352506683No Hit
CTACCGGCATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGGCTTC11700.11153947353368492No Hit
GACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATCACTGTCAGG11690.11144414065032279No Hit
AGCCAGGCCAGGCCCCTCTAGTAGTCGTCTATGATGATAGCGACCGGCCC11640.11096747623351219No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11610.1106814775834258No Hit
GCTTGGTCCCTCCGCCGAACACCCAATGATCACTACCGGCATCCCACACCTGACAGTGATAGTCGGCCTCATCCC11510.10972814874980458No Hit
GACTATCACTGTCAGGTGTGGGATGCCGGTAGTGATCATTGGGTGTTCGG11480.10944215009971821No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11230.1070588280156651No Hit
CCTGAGGGCCGGTCGCTATCATCATAGACGACTACTAGAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACA11150.10629616494876812No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA10920.10410350863143926No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC10900.10391284286471501No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG10710.10210151808083465No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTCTAGTAGTCGTCTATGATGAT10640.10143418789729979No Hit
GATCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT10570.10076685771376492No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG10510.10019486041359217No Hit
TCCCTGAGCGGTTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACC10490.10000419464686794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13900.042.2086261
GGTATCA5750.042.0143131
GTACTAA350.001345507939.442011
GTGGTAT2300.039.0132941
TGGTATC2400.038.796122
TATCAAC16050.036.9563562
ATCAACG16850.035.201753
TCAACGC17050.034.586574
CAACGCA17450.033.596135
AACGCAG18000.032.761176
GGGCCAG10100.031.73431270
CGTATGC450.00453034630.79316341
AGAGTAC19400.030.22063811
CGCAGAG19800.029.7828798
CGAGACT703.85302E-529.56881123
CAGAGTA20000.029.48645410
GCAGAGT20150.029.2669549
TTCGGGG8300.028.57617670
ACGCAGA20800.028.3510077
TACGGGT1750.027.58966415
Produced by FastQC (version 0.11.5)