FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048956
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGGCG55000.5243308584916813No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTACCGGCATCCCACAC47010.4481598846853443No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTA41240.3931528109853988No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40580.38686084068349863No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30410.2899072983042187No Hit
CTCTAGTAGTCGTCTATGATGATAGCGACCGGCCCTCAGGGATCCCTGAG29010.27656069463352134No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28370.2704593900983454No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27750.26454875132989375No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG27610.263214090962824No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC26700.25453879857687073No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26290.25063015035902364No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25250.24071553048936276No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24080.22956158313599426No Hit
ATATTACTGTGCGCGAGAATCCCGGGGCCACTGGGGGCAAAGAGGTCCGT23730.22622493221831994No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATGCTGACT23560.2246042732011638No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATGCTGACTCAGCCAC22720.21659631099874543No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22390.21345032584779533No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22170.21135300241382862No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21000.20019905506046012No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20500.19543241089235394No Hit
GCCCTCAGGGATCCCTGAGCGGTTCTCTGGCTCCAACTCTGGGAACACGG20470.19514641224226756No Hit
CAGTAATATAGGGCCGTGTCCGCAGCGGTCACAGAGCCCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCTA20430.19476508070881907No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT19660.18742444868993552No Hit
GGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAACCGCTCAGGGATCCCTGAGGGCCGGTCGCTATCATCATAGA19610.1869477842731249No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCACTAC18830.17951181937087923No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG18740.1786538234206201No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA18450.17588916980311853No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18150.1730291833022548No Hit
GGCTCTGTGACCTCCTATATGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGGCGGCCAGCATCAC18080.17236185311871993No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG17990.17150385716846084No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG17900.17064586121820172No Hit
GCACAGGCTCTGTGACCTCCTATATGCTGACTCAGCCACCCTCGGTGTCA17750.16921586796776986No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17540.16721387741716526No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17530.16711854453380315No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATATGCTGACTCAGC17460.16645121435026827No Hit
CTACTAGAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTTTTA17220.1641632251495773No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16650.15872925079793623No Hit
GTAATATAGGGCCGTGTCCGCAGCGGTCACAGAGCCCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCTACTGA16640.15863391791457412No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG16600.15825258638112563No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA16240.15482060258008914No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT15880.15138861877905269No Hit
CCCAATGATCACTACCGGCATCCCACACCTGACAGTGATAGTCGGCCTCA15640.14910062957836173No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC15610.14881463092827535No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA15360.14643130884422226No Hit
GTGTCAGTGGCCCCAGGAAAGGCGGCCAGCATCACCTGTGGGGGAAACAA15220.1450966484771525No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14660.13975800700887359No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14420.1374700178081826No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14400.13727935204145836No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14260.13594469167438863No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14170.1350866957241295No Hit
GCATCACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCT13750.13108271462292032No Hit
GTTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGG13740.13098738173955818No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATATGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGGCGG13670.13032005155602333No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13640.13003405290593695No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13620.1298433871392127No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13600.12965272137248846No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC13550.12917605695567783No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13520.12889005830559147No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13400.127746063705246No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13360.1273647321717975No Hit
CTATATTACTGTGCGCGAGAATCCCGGGGCCACTGGGGGCAAAGAGGTCCGTCCAACTGGTTCGACTCCTGGGGC13010.12402808125412314No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC12840.12240742223696703No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12690.12097742898653518No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12690.12097742898653518No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12640.12050076456972456No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12580.11992876726955183No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12510.11926143708601694No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGGCGGCCAGCATCACCTGTGGGGGAAACAACATTGGAAGTAAAAGTGTG12390.11811744248567146No Hit
GTGATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG12370.11792677671894722No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC12310.11735477941877447No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12100.11535278886816987No Hit
GGCCAGGCCCCTCTAGTAGTCGTCTATGATGATAGCGACCGGCCCTCAGG12080.11516212310144563No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11950.11392279561773802No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTCCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC11700.11153947353368492No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11680.11134880776696068No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT11560.11020481316661519No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11290.10763082531583784No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11280.1075354924324757No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11270.1074401595491136No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTCTAGTAGTCGTCTATGATGAT11260.10734482666575147No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11200.10677282936557872No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGATCA11120.10601016629868174No Hit
CTACCGGCATCCCACACCTGACAGTGATAGTCGGCCTCATCCCCGGCTTC11060.105438168998509No Hit
GACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTATCACTGTCAGG11040.10524750323178474No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11030.10515217034842263No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT10980.10467550593161201No Hit
CTGCACAGGCTCTGTGACCTCCTATATGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGGCGGCCAG10920.10410350863143926No Hit
GCTTGGTCCCTCCGCCGAACACCCAATGATCACTACCGGCATCCCACACCTGACAGTGATAGTCGGCCTCATCCC10910.10400817574807715No Hit
GACTATCACTGTCAGGTGTGGGATGCCGGTAGTGATCATTGGGTGTTCGG10860.10353151133126652No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC10780.10276884826436954No Hit
AGCCAGGCCAGGCCCCTCTAGTAGTCGTCTATGATGATAGCGACCGGCCC10560.1006715248304028No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10510.10019486041359217No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTA150.002323013569.24197440
TACGAAA300.002877208465.59997670
GTGGTAT2250.045.9191441
GGTATCA7000.042.8032041
GTATCAA17250.042.3254741
TGGTATC2650.038.967512
TATCAAC18850.038.347342
ATCAACG19900.036.3239863
CAACGCA20350.035.351615
TCAACGC20500.035.260854
AACGCAG20600.035.0896766
CGCAGAG21950.032.6350178
AGAGTAC21450.032.43395211
CAGAGTA21950.031.85204310
TACACCG652.357851E-531.7735085
ACGCAGA22550.031.7606247
GGGCCAG10150.031.66895370
GCAGAGT22900.031.1322619
CTTATAT450.004674436530.5967122
TATACTC802.7152455E-630.2527537
Produced by FastQC (version 0.11.5)