FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548599
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT6910.1259572110047594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAT350.00652595453.33680370
GTATCAA18250.039.3168951
GGTATCA7750.038.5401461
GTGGTAT3150.037.0564771
TATCAAC20250.035.7434732
TATACAC1650.035.3430753
TAGACGG501.92362E-434.3035745
TGGTATC3350.031.7436052
TCAACGC22750.031.6648394
ATCAACG22800.031.5953983
CAACGCA22850.031.526265
AACGCAG23100.031.1850686
TAACGGC450.004751473730.49206413
AGAGTAC24250.029.28181311
ACGCAGA24700.029.1649827
CAGAGTA24500.028.98301910
CTCGTAT1700.028.56504839
CTTATAC1950.028.169631
TCGTATG1850.028.15502540
CGCAGAG25600.028.139658
Produced by FastQC (version 0.11.5)