FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548599
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15580.28399614290219266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19600.036.0568431
GGTATCA9150.034.119041
GTGGTAT4300.031.1152081
TATCAAC22650.030.890132
TGGTATA450.004755841630.4863362
AACGCAG24250.029.415066
AGAGTAC23850.028.62994211
CAGAGTA23900.028.57004710
CAACGCA24800.028.2095835
TCAACGC24850.028.1528244
TGGTATC4400.028.0612852
ACGCAGA25700.027.4935917
ATCAACG25450.027.4891033
CGCAGAG25800.027.259058
GCAGAGT25900.027.0262729
GAGTACG26950.022.79029812
AGTACGG26800.022.6617913
GTATTAG1154.8005622E-520.8822251
TACGGGT4300.019.15131415
TACGGGG11650.019.14446315
Produced by FastQC (version 0.11.5)