FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005755061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005755061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335663
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT6370.18977367180773572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5050.046.2051771
GTATCAA12300.040.172651
ATTACGA557.665198E-637.4280325
AGGGGGG1553.261448E-936.1438370
TATACAC1051.6370905E-1135.9427873
TCTTATA501.9202396E-434.309032
TTGACAC400.002665293934.309033
TATCAAC14650.033.4893572
GTGGTAT1600.032.1695061
CGTATGC1207.094059E-1131.82925841
TCGTATG1207.2759576E-1131.7765640
CTCGTAT1207.4578566E-1131.74788939
ATCAACG15500.031.4314943
CGGAATT800.00429060831.1238570
GTATGCC1359.094947E-1230.89267742
AACGCAG15800.030.6175526
TCAACGC15850.030.5209664
CCTAATT450.004741725530.50145715
CAACGCA15950.030.3296095
TGGTATC1600.030.02042
Produced by FastQC (version 0.11.5)